Anti-CTRP5 抗体 (ab36893)
Key features and details
- Rabbit polyclonal to CTRP5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CTRP5 antibody -
製品の詳細
Rabbit polyclonal to CTRP5 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A 15 amino acid peptide from near the amino terminus of human CTRP5.
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ポジティブ･コントロール
- Human brain cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36893の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa).
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| IHC-P | (3) |
Use a concentration of 10 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 25 kDa). |
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IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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関連疾患
Late-onset retinal degeneration -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 114902 Human
- Omim: 605670 Human
- SwissProt: Q9BXJ0 Human
- Unigene: 632102 Human
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別名
- C1q and tumor necrosis factor related protein 5 antibody
- C1QT5_HUMAN antibody
- C1qtnf5 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)This image is courtesy of an Abreview submitted by Steffen Rickelt
ab36893 staining CTRP5 in human pancreas carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/750 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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Western blot - Anti-CTRP5 antibody (ab36893)Lane 1 : Anti-CTRP5 antibody (ab36893) at 1 µg/ml
Lane 2 : Anti-CTRP5 antibody (ab36893) at 2 µg/ml
Lane 3 : Anti-CTRP5 antibody (ab36893) at 4 µg/ml
All lanes : Human brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : Anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 25 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Lanes read from left to right, 1-3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP5 antibody (ab36893)ab36993 at 10µg/ml staining CTRP5 in human brain tissue by IHC
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab36893 は論文での使用が確認できていません。