Anti-CTRP4 抗体 (ab36871)
Key features and details
- Rabbit polyclonal to CTRP4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-CTRP4 antibody -
製品の詳細
Rabbit polyclonal to CTRP4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Rat -
免疫原
A 16 amino acid peptide from near the carboxy terminus of human CTRP4.
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ポジティブ・コントロール
- Rat brain cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36871の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
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IHC-P |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
May act as a pro-inflammatory cytokine, that induces the activation of NF-kappa-B signaling pathway and up-regulates IL6 production. -
組織特異性
Widely expressed at low levels. -
配列類似性
Contains 2 C1q domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C1q and tumor necrosis factor related protein 4 antibody
- C1QT4_HUMAN antibody
- C1qtnf4 antibody
see all
画像
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Lane 1 : Anti-CTRP4 antibody (ab36871) at 1 µg/ml
Lane 2 : Anti-CTRP4 antibody (ab36871) at 2 µg/ml
Lane 3 : Anti-CTRP4 antibody (ab36871) at 4 µg/ml
All lanes : Rat brain cell lysate
Lysates/proteins at 15 µg/ml per lane.
Secondary
All lanes : anti-rabbit IgG HRP at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Lanes read from left to right 1, 2 and 3. These proteins are often highly modified post-translationally and may migrate in SDS-PAGE at positions other than their predicted size. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTRP4 antibody (ab36871)
ab36891 at 10µg/ml staining CTRP4 in rat brain by IHC
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Immunofluorescence of CTRP4 in Rat Brain cells using ab36871 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab36871 は 4 報の論文で使用されています。
- Vester SK et al. Nucleolin acts as the receptor for C1QTNF4 and supports C1QTNF4-mediated innate immunity modulation. J Biol Chem 296:100513 (2021). PubMed: 33676896
- Li Q et al. Ctrp4, a new adipokine, promotes the differentiation of osteoblasts. Biochem Biophys Res Commun 512:224-229 (2019). PubMed: 30885436
- Bando H et al. CXCR4 regulates Plasmodium development in mouse and human hepatocytes. J Exp Med 216:1733-1748 (2019). PubMed: 31189656
- Byerly MS et al. C1q/TNF-related protein 4 (CTRP4) is a unique secreted protein with two tandem C1q domains that functions in the hypothalamus to modulate food intake and body weight. J Biol Chem 289:4055-69 (2014). WB ; Mouse . PubMed: 24366864