Anti-Ctip2 抗体 (ab70453)
Key features and details
- Rabbit polyclonal to Ctip2
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Ctip2 antibody
Ctip2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ctip2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
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免疫原
Synthetic peptide corresponding to a region between C terminal residues 850-894 of human Ctip2 (Swiss-Prot Q9C0K0).
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ポジティブ･コントロール
- WB: Jurkat, MOLT-4 cells; IP:Jurkat; IHC: human prostate carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70453の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa).
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| IP |
Use at 2-10 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 120-130 kDa (predicted molecular weight: 100 kDa). |
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IP
Use at 2-10 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. May be a key regulator of both differentiation and survival during thymocyte development. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. -
組織特異性
Highly expressed in brain and in malignant T-cell lines derived from patients with adult T-cell leukemia/lymphoma. -
配列類似性
Contains 6 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64919 Human
- Omim: 606558 Human
- SwissProt: Q9C0K0 Human
- Unigene: 709690 Human
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別名
- ATL1 alpha antibody
- ATL1 antibody
- ATL1 beta antibody
see all
画像
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Western blot - Anti-Ctip2 antibody (ab70453)All lanes : Anti-Ctip2 antibody (ab70453) at 0.1 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Lane 4 : MOLT-4 whole cell lysate at 50 µg
Lane 5 : MOLT-4 whole cell lysate at 15 µg
Predicted band size: 100 kDa
Exposure time: 3 minutesPrepared using NETN lysis
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Immunoprecipitation - Anti-Ctip2 antibody (ab70453)Ctip2 was immunoprecipitated from Jurkat whole cell lysate (1 mg per IP reaction, 20% loaded) with ab70453 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab70453 at 1 µg/mL.
Lane 1: rabbit anti-Bcl11b antibody IP in Jurkat whole cell lysate.
Lane 2: ab70453 IP in Jurkat whole cell lysate.
Lane 3: Control IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody (ab70453)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ctip2 with ab70453 at 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab70453 は 5 報の論文で使用されています。
- Sanders B et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
- Marcilla D et al. (Immuno)histological Analysis of Ewing Sarcoma. Methods Mol Biol 2226:49-64 (2021). PubMed: 33326093
- Orth MF et al. High Specificity of BCL11B and GLG1 for EWSR1-FLI1 and EWSR1-ERG Positive Ewing Sarcoma. Cancers (Basel) 12:N/A (2020). PubMed: 32164354
- Forbes LH & Andrews MR Grafted Human iPSC-Derived Neural Progenitor Cells Express Integrins and Extend Long-Distance Axons Within the Developing Corticospinal Tract. Front Cell Neurosci 13:26 (2019). PubMed: 30809126
- Baldauf MC et al. Robust diagnosis of Ewing sarcoma by immunohistochemical detection of super-enhancer-driven EWSR1-ETS targets. Oncotarget 9:1587-1601 (2018). PubMed: 29416716