Anti-Ctip2 抗体 [25B6]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (AB18465)

IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse hippocampus performed on a Leica Biosystems BOND^® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (AB18465)

Negative control image : IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human cerebellum* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• Flow Cyt

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Flow Cytometry - Anti-Ctip2 antibody [25B6] (AB18465)

Overlay histogram showing Jurkat cells stained with ab18465 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18465 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rat IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG (2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5000 events was performed.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (AB18465)

Immunofluorescence staining of Ctip2 using ab18465 in Jurkat cells (+ve expression control top panel) and Daudi cells (-ve expression control bottom panel). The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% Triton-X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab18465 at 0.2 µg/mL and ab6046 at 1 µg/mL overnight at +4°C followed by a further incubation at room temperature for 1h with Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150165) (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150084) (shown in red) both at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (AB18465)

Immunofluorescence staining of Ctip2 using ab18465 in Jurkat cells (+ve expression control top panel) and Daudi cells (-ve expression control bottom panel). The cells were fixed with 100% methanol (5 min) permeabilized with 0.1% Triton-X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab18465 at 0.2 µg/mL and ab6046 at 1 µg/mL overnight at +4°C followed by a further incubation at room temperature for 1h with Goat Anti-Rat IgG H&L (Alexa Fluor^® 488) preadsorbed (ab150165) (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed (ab150084) (shown in red) both at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (AB18465)

IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded human hippocampus* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (AB18465)

Neonatal Mouse Hippocampal Neurons (Harvested at P1 grown 5d in culture on glial cell feeder layer).

Red is beta tubulin staining.
Green is ab18465.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Ctip2 antibody [25B6] (AB18465)

Neonatal mouse hippocampal neurons stained with ab18465 (top panel - green) and Ctip1 antibody (middle - red). Bottom panel is overlay of ab18465 and Ctip1 antibody staining - yellow indicates co-localisation green is ab18465 alone and red is Ctip1 antibody alone.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip2 antibody [25B6] (AB18465)

Negative control image : IHC image of Ctip2 staining in a section of formalin-fixed paraffin-embedded mouse cerebellum performed on a Leica Biosystems BOND^® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab18465, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

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Western blot - Anti-Ctip2 antibody [25B6] (AB18465)

Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody.

Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

Western blot using ab18465 on nuclear extract from Jurkat cells immunoprecipitated with anti-Sir2 antibody. Two bands are seen which may correspond to two CTIP2 transcripts present in Jurkat cells as previously reported (Bernard et al. 2001).

All lanes:

Western blot - Anti-Ctip2 antibody [25B6] (ab18465)

Predicted band size: 96 kDa

false

• WB

AbReview24135****

Western blot - Anti-Ctip2 antibody [25B6] (AB18465)

All lanes:

Western blot - Anti-Ctip2 antibody [25B6] (ab18465) at 1/500 dilution

Lanes 1 - 2:

Mouse brain tissue lysate at 1.5 µg

Lane 3:

Mouse brain tissue lysate at 3 µg

Secondary

All lanes:

IRDYE 680-conjugated Donkey Anti-Rat polyclonal. at 1/10000 dilution

Predicted band size: 96 kDa

Observed band size: 100 kDa,110 kDa

false

Exposure time: 10min

This image is courtesy of an Anonymous Abreview.