AB191401

Anti-Ctip1/BCL-11A 抗体 [EPR14943-44]

Anti-Ctip1/BCL-11A antibody [EPR14943-44]

Advanced Validation
RabMAb
Recombinant
KO Validated
20ul selling size
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(12 Publications)

Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) is a rabbit monoclonal antibody detecting Ctip1/BCL-11A in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF, ChIC/CUT&RUN sequencing. Suitable for Human, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

CTIP1, EVI9, KIAA1809, ZNF856, BCL11A, BCL11 transcription factor A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, BCL-11A, EVI-9

11 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunohistochemical analysis of paraffin-embedded, Human tonsil tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Ctip1/BCL-11A with purified ab191401 at 1/200 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Unknown

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ab191401 (purified) at 1/500 dilution (2.1 © : g/ml) immunoprecipitating Ctip1 / BCL-11A in Raji whole cell lysate.
Lane 1 (input) : Raji(Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10© : g
Lane 2 (+) : ab191401 & Raji whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab191401 in Raji whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM /TBST .

All lanes:

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

Predicted band size: 91 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunofluorescence analysis of, paraformaldehyde-fixed, rat C6 cells labeling Ctip1/BCL-11A with ab191401 at a 1/450 dilution (4 ug/ml). As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/200 dilution. In blue DAPI staining. In the negative controls cells were treated with anti-BCL11A at a 1/450 dilution as primary antibody and goat anti-mouse IgG (Alexa Fluor®594) at a 1/400 dilution as secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling Ctip1/BCL-11A with ab191401 at a 1/100 dilution (19 μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : Empty
Lane 3 : BCL11A (Ctip1) knockout HAP1 whole cell lysate (20 μg)
Lane 4 : Raji whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab191401 observed at 91 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab191401 was shown to recognize BCL11A (Ctip1) in wild type cells as signal was lost at the expected MW in BCL11A (Ctip1) knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BCL11A (Ctip1) knockout samples were subjected to SDS-PAGE. ab191401 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401)

Predicted band size: 91 kDa

false

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution

All lanes:

293 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

true

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 10^5 Raji (human Burkitt's lymphoma B lymphocyte) cells and 5 µg of ab191401 [EPR14943-44]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN-seq

Lab

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) at 1/50000 dilution

Lane 1:

Raji cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 91 kDa

Observed band size: 120 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR14943-44

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

IHC-P, WB, ChIC/CUT&RUN-seq, IP, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples. Please see images below for suitable positive controls.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/200", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ChICCUTRUNseq-species-checked": "predicted", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/450", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "" } } }

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製品の詳細

What is this antibody validated in?
Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) and ChIC/CUT&RUN sequencing in Human, Rat samples.

What is the molecular weight of Ctip1/BCL-11A?
Anti-Ctip1/BCL-11A [EPR14943-44] (ab191401) specifically detects a band for Ctip1/BCL-11A (UniProt: Q9H165) at a molecular weight of 91kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Ctip1/BCL-11A antibody [EPR14943-44] (ab191401) has been confirmed by Western blot testing in BCL11A Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR14943-44] also available for your convenience: ab191401, Carrier free - ab242406

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcription factor (PubMed : 16704730, PubMed : 29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed : 23644491, PubMed : 39607926). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed : 29606353, PubMed : 39423807). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed : 26375765, PubMed : 29606353, PubMed : 39423807, PubMed : 39607926). Involved in brain development (PubMed : 27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).

See full target information BCL11A

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文献 (12)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30552 PubMed40836054

2025

Adipocyte-derived factors induce adherent to suspension transition in breast and pancreatic cancer cells through lipid metabolic alteration.

Applications

Unspecified application

Species

Unspecified reactive species

Doru Kwon,Hyun Woo Park,Byung Soh Min,Junjeong Choi

Nature communications 16:6919 PubMed40715076

2025

Removal of promoter CpG methylation by epigenome editing reverses HBG silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Henry W Bell,Ruopeng Feng,Manan Shah,Yu Yao,James Douglas,Phillip A Doerfler,Thiyagaraj Mayuranathan,Michael F O'Dea,Yichao Li,Yong-Dong Wang,Jingjing Zhang,Joel P Mackay,Yong Cheng,Kate G R Quinlan,Mitchell J Weiss,Merlin Crossley

The EMBO journal 44:3448-3467 PubMed40328966

2025

CHD8 interacts with BCL11A to induce oncogenic transcription in triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mark Waterhouse,Kyren Lazarus,Maria Francesca Santolla,Sara Pensa,Eleanor Williams,Abigail J Q Siu,Hisham Mohammed,Irina Mohorianu,Marcello Maggiolini,Jason Carroll,Laura S Itzhaki,Taufiq Rahman,Walid T Khaled

EMBO molecular medicine 15:e17810 PubMed37807875

2023

The transcription factor HIF2α partakes in the differentiation block of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Magliulo,Matilde Simoni,Carolina Caserta,Cristina Fracassi,Serena Belluschi,Kety Giannetti,Raffaella Pini,Ettore Zapparoli,Stefano Beretta,Martina Uggè,Eleonora Draghi,Federico Rossari,Nadia Coltella,Cristina Tresoldi,Marco J Morelli,Raffaella Di Micco,Bernhard Gentner,Luca Vago,Rosa Bernardi

Cancer biology & therapy 24:2235768 PubMed37548553

2023

A feedback loop between lncRNA MALAT1 and DNMT1 promotes triple-negative breast cancer stemness and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Hu,Yuqiong He,Na Luo,Xin Li,Lei Guo,Kejing Zhang

Cancers 14: PubMed36230873

2022

CircKIF4A Is a Prognostic Factor and Modulator of Natural Killer/T-Cell Lymphoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Rongfang He,Wei Wen,Bibo Fu,Renjie Zhu,Guanjun Chen,Shenrui Bai,Xi Cao,Hua Wang

Cell chemical biology 29:1273-1287.e8 PubMed35839780

2022

Temporal resolution of gene derepression and proteome changes upon PROTAC-mediated degradation of BCL11A protein in erythroid cells.

Applications

Unspecified application

Species

Unspecified reactive species

Stuti Mehta,Altantsetseg Buyanbat,Yan Kai,Ozge Karayel,Seth Raphael Goldman,Davide Seruggia,Kevin Zhang,Yuko Fujiwara,Katherine A Donovan,Qian Zhu,Huan Yang,Behnam Nabet,Nathanael S Gray,Matthias Mann,Eric S Fischer,Karen Adelman,Stuart H Orkin

eLife 10: PubMed34585664

2021

3'HS1 CTCF binding site in human β-globin locus regulates fetal hemoglobin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela Himadewi,Xue Qing David Wang,Fan Feng,Haley Gore,Yushuai Liu,Lei Yu,Ryo Kurita,Yukio Nakamura,Gerd P Pfeifer,Jie Liu,Xiaotian Zhang

Nature genetics 53:511-520 PubMed33649594

2021

Transcription factor competition at the γ-globin promoters controls hemoglobin switching.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liu,Shuqian Xu,Qiuming Yao,Qian Zhu,Yan Kai,Jonathan Y Hsu,Phraew Sakon,Luca Pinello,Guo-Cheng Yuan,Daniel E Bauer,Stuart H Orkin

Oncology letters 19:2916-2924 PubMed32218847

2020

BCL11A confers cell invasion and migration in androgen receptor-positive triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Yumei Xu,Kun Xu,Yajuan Chen,Xiudi Xiao,Xiaoxiang Guan

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ctip1/BCL-11A 抗体 [EPR14943-44]

Anti-Ctip1/BCL-11A antibody [EPR14943-44]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunocytochemistry/ Immunofluorescence - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

ChIC/CUT&RUN sequencing - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

Western blot - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (12)

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Abcam product promise