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AB264423

Anti-Ctip1/BCL-11A 抗体 [BLR073G] - BSA free

Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free

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Rabbit Recombinant Monoclonal Ctip1/BCL-11A antibody. Suitable for IP, WB, Flow Cyt and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human BCL11A aa 1-50.

別名を表示する

CTIP1, EVI9, KIAA1809, ZNF856, BCL11A, BCL11 transcription factor A, B-cell CLL/lymphoma 11A, B-cell lymphoma/leukemia 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, BCL-11A, EVI-9

4 Images
Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human Bcl11a with ab264423 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • IP

Supplier Data

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Ctip1/BCL-11A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab264423 at 6 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab264423 at 1/1000 dilution.

Lane 1 : ab264423 IP in Ramos whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 75 secs.

All lanes:

Immunoprecipitation - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423)

Predicted band size: 91 kDa

false

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • WB

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1:

Ramos whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

MOLT4 whole cell lysate at 50 µg

Lane 5:

U-2 OS whole cell lysate at 50 µg

Lane 6:

Raji whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 91 kDa

false

Exposure time: 3min

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)
  • WB

Supplier Data

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (AB264423)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Ctip1/BCL-11A antibody [BLR073G] - BSA free (ab264423) at 1/1000 dilution

Lane 1:

BW5147.3 whole cell lysate at 50 µg

Lane 2:

A20 whole cell lysate at 50 µg

Lane 3:

RenCa whole cell lysate at 50 µg

Lane 4:

EL4 whole cell lysate at 50 µg

Lane 5:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 91 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

BLR073G

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

Flow Cyt, WB, IP

applications

免疫原

Synthetic Peptide within Human BCL11A aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H165

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代替製品

Primary Antibodies

AB19487

Anti-Ctip1/BCL-11A antibody [14B5]

KO Validated

Western blot - Anti-Ctip1/BCL-11A antibody [14B5] (AB19487)

  • 3
  • 2
Primary Antibodies

AB19489

Anti-Ctip1/BCL-11A antibody [18B12DE6]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [18B12DE6] (AB19489)

  • 4
  • 3
Primary Antibodies

AB242406

Anti-Ctip1/BCL-11A antibody [EPR14943-44] - BSA and Azide free

Advanced Validation|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] - BSA and Azide free (AB242406)

  • 0
  • 0
Primary Antibodies

AB191401

Anti-Ctip1/BCL-11A antibody [EPR14943-44]

Advanced Validation|RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip1/BCL-11A antibody [EPR14943-44] (AB191401)

  • 0
  • 0
Primary Antibodies

AB256127

Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free

BOND RX™ Validated|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ctip1/BCL-11A antibody [123] - BSA and Azide free (AB256127)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB187156

Anti-USP25 antibody [EPR15019]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-USP25 antibody [EPR15019] (AB187156)

  • 0
  • 0
Primary Antibodies

AB240636

Anti-Ctip2 antibody [EPR23120-25]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

  • 5
  • 1
Primary Antibodies

AB134970

Anti-ZPR1 antibody [EPR7595]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZPR1 antibody [EPR7595] (AB134970)

  • 0
  • 0
Primary Antibodies

AB181105

Anti-USP4 antibody [EPR13846]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-USP4 antibody [EPR13846] (AB181105)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>6 μl/mg lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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製品の詳細

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Purified from TCS.
バッファー組成
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
+4°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Transcription factor (PubMed : 16704730, PubMed : 29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed : 23644491, PubMed : 39607926). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed : 29606353, PubMed : 39423807). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed : 26375765, PubMed : 29606353, PubMed : 39423807, PubMed : 39607926). Involved in brain development (PubMed : 27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity).
See full target information BCL11A
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Abcam product promise

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