Anti-CtIP 抗体 (ab70163)
Key features and details
- Rabbit polyclonal to CtIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CtIP antibody
CtIP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CtIP -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig, Chimpanzee, Baboon, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human CtIP aa 850-897 (C terminal). The exact sequence is proprietary. (NP_002885.1).
Database link: Q99708 -
ポジティブ･コントロール
- MCF7 and HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70163の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 102 kDa).
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| IP |
Use at 2 µg/mg of lysate.
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 102 kDa). |
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IP
Use at 2 µg/mg of lysate. |
ターゲット情報
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機能
Endonuclease that cooperates with the MRE11-RAD50-NBN (MRN) complex in processing meiotic and mitotic double-strand breaks (DSBs) by ensuring both resection and intrachromosomal association of the broken ends. Functions downstream of the MRN complex and ATM, promotes ATR activation and its recruitment to DSBs in the S/G2 phase facilitating the generation of ssDNA. Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage. Promotes microhomology-mediated alternative end joining (A-NHEJ) during class-switch recombination and plays an essential role in chromosomal translocations. -
関連疾患
Seckel syndrome 2
Jawad syndrome
Genetic variability in RBBP8 is noted as a factor in BRCA1-associated breast cancer risk (PubMed:21799032). Exhibits sensitivity to tamoxifen in certain breast cancer cell lines (PubMed:18171986). -
配列類似性
Belongs to the COM1/SAE2/CtIP family. -
ドメイン
The PXDLS motif binds to a cleft in CtBP proteins.
The damage-recruitment motif is required for DNA binding and translocation to sites of DNA damage. -
翻訳後修飾
Acetylated. Deacetylation by SIRT6 upon DNA damage promotes DNA end resection.
Hyperphosphorylation upon ionizing radiation results in dissociation from BRCA1. Phosphorylation at Thr-847 by CDK1 is essential for the recruitment to DNA and the DNA repair function. Phosphorylated on Ser-327 as cells enter G2 phase. This phosphorylation is required for binding BRCA1 and for the G2/M DNA damage transition checkpoint control.
Ubiquitinated (PubMed:14654780, PubMed:16818604). Ubiquitination at multiple sites by BRCA1 (via its N-terminal RING domain) does not lead to its proteosomal degradation but instead the ubiquitinated RBBP8 binds to chromatin following DNA damage and may play a role in G2/M checkpoint control (PubMed:16818604). Ubiquitinated by RNF138 at its N-terminus (PubMed:26502057). -
細胞内局在
Nucleus. Chromosome. Associates with sites of DNA damage in S/G2 phase (PubMed:10764811). Ubiquitinated RBBP8 binds to chromatin following DNA damage (PubMed:16818604). - Information by UniProt
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参照データベース
- Entrez Gene: 5932 Human
- Omim: 604124 Human
- SwissProt: Q99708 Human
- Unigene: 546282 Human
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別名
- COM1 antibody
- COM1_HUMAN antibody
- CtBP interacting protein antibody
see all
画像
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Western blot - Anti-CtIP antibody (ab70163)All lanes : Anti-CtIP antibody (ab70163) at 1 µg/ml
Lane 1 : MCF7 whole cell lysate at 50 µg
Lane 2 : MCF7 whole cell lysate at 15 µg
Lane 3 : MCF7 whole cell lysate at 5 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Predicted band size: 102 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-CtIP antibody (ab70163)CtIP was immunoprecipitated from 293T cells using NETN lysis buffer with ab70163 at 2ug/mg of lysate.
Chemiluminescence with an exposure time: 3 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab70163 は 12 報の論文で使用されています。
- Wang LM et al. Thioparib inhibits homologous recombination repair, activates the type I IFN response, and overcomes olaparib resistance. EMBO Mol Med 15:e16235 (2023). PubMed: 36652375
- Zhang W et al. A high-throughput small molecule screen identifies farrerol as a potentiator of CRISPR/Cas9-mediated genome editing. Elife 9:N/A (2020). PubMed: 32644042
- Hustedt N et al. Control of homologous recombination by the HROB-MCM8-MCM9 pathway. Genes Dev 33:1397-1415 (2019). PubMed: 31467087
- Batenburg NL et al. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection. Nucleic Acids Res 47:10678-10692 (2019). PubMed: 31501894
- Saquilabon Cruz GM et al. Femtosecond near-infrared laser microirradiation reveals a crucial role for PARP signaling on factor assemblies at DNA damage sites. Nucleic Acids Res 44:e27 (2016). ICC/IF . PubMed: 26424850