Anti-CTGF 抗体

• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-CTGF antibody (AB6992)

CTGF Immunohistochemistry-immunofluorescence using Anti-CTGF antibody ab6992. Publication image and figure legend from Li, C., Zhen, G., et al., 2016, Nat Commun, PubMed 27126736.

Activation of RhoA promotes CTGF-VEGF complex formation in ECM, whereas inactivation of RhoA induces MMP3-mediated CTGF cleavage and VEGF release.(a-d) Immunofluorescence analysis of the binding of VEGF to ECM in different MSC cultures. MSCs were incubated with the indicated treatments for 7 days. Immunofluorescence staining was performed on non-permeabilized cells using antibodies against CTGF (a), fibronectin (b), collagen I (c) or VEGF (d). (e,f) MSCs were transfected with siRNA-control or siRNA-CTGF, then cultured with the indicated medium for 6 days. Western blot (WB) analysis of the cell lysates was performed using antibodies against CTGF and β-actin (e). Immunofluorescence staining was performed on non-permeabilized cells using antibodies against CTGF or VEGF (f). (g-j) WB and co-immunoprecipitation (IP) analyses of VEGF and CTGF in the overlay media of MSCs with different treatment. MSCs were incubated with the indicated treatments for 7 days. Overlay media : M1, M2, M3 and M4 were collected, respectively. Western blotting analysis of the overlay media was performed using antibodies against CTGF (g), MMP3 (h) and VEGF (i). Overlay media were subjected to IP assays using antibody against VEGF, the VEGF-associated CTGF was detected by western blotting with antibody against CTGF (j). (k,l) WB analysis of VEGF and CTGF in the ECMs of the cultured MSCs with different treatment. MSCs were incubated with the indicated treatment for 7 days. ECMs : E1, E2, E3 and E4 were collected. Western blotting analysis of the ECMs was performed using antibodies against CTGF (k) and VEGF (l). (m-o) WB and co-IP analysis of VEGF and CTGF in the overlay media of MSCs transfected with empty vector (EV) or L63RhoA. In the aliquots of the overlay medium collected from the cells overexpressed L63RhoA, 50 or 100 ng ml-1 rMMP3 was added and the reactions were maintained at 37 °C for 2 h. Overlay media were subjected to immunoprecipitation assays using antibody against VEGF, the VEGF-associated CTGF was detected by western blotting with antibody against CTGF (m). Western blotting analysis of the overlay media was performed using antibodies against CTGF (n) and MMP3 (o). Scale bars, 50 μm. Data are representative of three independent experiments.

• WB

CiteAb

Western blot - Anti-CTGF antibody (AB6992)

CTGF western blot using Anti-CTGF antibody ab6992. Publication image and figure legend from Choi, S. Y., Bae, H., et al., 2020, Nat Commun, PubMed 31980640.

FRC-specific depletion of Ltbr activates YAP/TAZ-induced myofibrosis.a Diagram for generation of indicated mice and their analyses at 8-weeks old. b Representative images and comparisons of indicated marker expressions on CCL19-YFP+ FRCs in WT^ΔFRC-YR and Ltbr^ΔFRC-YR mice. Scale bars, 20 µm. c Representative images and comparisons of YAP and TAZ nuclear localization (white arrows) in inguinal LN of WT^ΔFRC and Ltbr^ΔFRC mice. Scale bars, 20 µm. d Comparison of indicated mRNA expression in FRCs sorted from WT^ΔFRC and Ltbr^ΔFRC mice. Each dot indicates a mean of quadruplicate values using n = 8-12 mice/group from three independent experiments. e Diagram for primary culture of FRCs derived from i-Lats1/2^ΔFRC-TR mice and treatment with EtOH (control) or 4-OHT at 4 days after the culture and their analyses at 2 days after the treatment. f Immunoblot analysis of indicated proteins in primary cultured mouse FRCs after treatment with EtOH or 4-OHT for 2 days. g Comparisons of indicated mRNA expression normalized to Gapdh in primary cultured mouse FRCs after treatment with EtOH or 4-OHT for 2 days. Each dot indicates a mean of triplicate values from three independent experiments. h, i Representative images and comparisons of indicated marker expressions in primary cultured mouse FRCs after treatment with EtOH or 4-OHT for 2 days. Scale bars, 30 μm. Each dot indicates a mean of triplicate values from three independent experiments. j Diagram for primary culture of human FRCs for 4 days and infection with an adenovirus to induce overexpression of active YAP (YAP5SA) or TAZ (TAZ4SA) for their analyses at 2 days after the infection. k, l Representative images and comparisons of indicated marker expressions in primary cultured human FRCs infected with control-, YAP5SA-, or TAZ4SA-adenovirus. Scale bars, 30 μm. Each dot indicates a mean of triplicate values from three independent experiments. Unless otherwise denoted, each dot indicates a value obtained from one mouse and n = 5 mice/group pooled from two independent experiments. Horizontal bars indicate mean ± SD and P values versus WT^ΔFRC or WT^ΔFRC-YRby two-tailed Mann-Whitney U test except for (g), (i), and (l) (two-tailed Student's t-test). NS, not significant.

false

• WB

CiteAb

Western blot - Anti-CTGF antibody (AB6992)

CTFG western Blot using Anti-CTGF antibody ab6992. Publication image and figure legend from Cheong, M. L., Lai, T. H., et al. 2019, PLoS One PubMed 30695033.

Effects of TGFβ isoforms on collagen and CTGF protein and mRNA expression in HESCs.(A) Cells were treated with the indicated concentration of TGFβ1 for 16 h and then analyzed through Western blotting (n = 3). (B) Effect of TGFβ isoforms on collagen and CTGF mRNA expression in HESCs. Cells were treated with vehicle or TGFβ isoforms (ng/ml) for 4 h. The mRNA expression level of collagen, CTGF, and β-actin was analyzed through RT-PCR and densitometry (n = 2). *p < < 0.05, **p < < 0.01, and ***p < < 0.001 versus vehicle treatment only (basal).

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