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Anti-CTGF 抗体 (ab5097)

Reviews (9)Q&A (18)References (23)
Immunocytochemistry - Anti-CTGF antibody (ab5097)
  • Western blot - Anti-CTGF antibody (ab5097)
  • Immunocytochemistry - Anti-CTGF antibody (ab5097)

Key features and details

  • Rabbit polyclonal to CTGF
  • Suitable for: ICC, IHC-Fr, WB, IHC-P, ICC/IF
  • Reacts with: Rat, Human, Pig, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-CTGF antibody [EPR20728] (ab209780)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-CTGF antibody
    CTGF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CTGF

  • 由来種

    Rabbit

  • 特異性

    This antibody detects recombinant human CTGF by Western Blotting. The band can be blocked by the immunising peptide (ab13741). We have been unable to detect endogenous protein in WB with ab5097.

  • アプリケーション

    適用あり: ICC, IHC-Fr, WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human, Pig, Recombinant fragment

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 98.98% PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5097の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use a concentration of 1 µg/ml.
IHC-Fr (2)
Use at an assay dependent concentration.
WB
1/500. Predicted molecular weight: 38 kDa.

This application has only been tested on recombinant protein.
IHC-P (6)
Use at an assay dependent concentration. PubMed: 17028989
ICC/IF (1)
Use at an assay dependent concentration.
特記事項
ICC
Use a concentration of 1 µg/ml.
IHC-Fr
Use at an assay dependent concentration.
WB
1/500. Predicted molecular weight: 38 kDa.

This application has only been tested on recombinant protein.
IHC-P
Use at an assay dependent concentration. PubMed: 17028989
ICC/IF
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

  • 組織特異性

    Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

  • 配列類似性

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix. Secreted.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CCN 2 antibody
    • CCN family member 2 antibody
    • CCN2 antibody
    • Connective tissue growth factor antibody
    • Ctgf antibody
    • CTGF_HUMAN antibody
    • Hcs 24 antibody
    • Hcs24 antibody
    • Hypertrophic chondrocyte specific protein 24 antibody
    • Hypertrophic chondrocyte-specific gene product 24 antibody
    • Hypertrophic chondrocyte-specific protein 24 antibody
    • IBP-8 antibody
    • IGF-binding protein 8 antibody
    • IGFBP-8 antibody
    • IGFBP8 antibody
    • Insulin like Growth Factor Binding Protein 8 antibody
    • Insulin-like growth factor-binding protein 8 antibody
    • MGC102839 antibody
    • NOV 2 antibody
    • NOV2 antibody
    see all

画像

  • Immunocytochemistry - Anti-CTGF antibody (ab5097)
    Immunocytochemistry - Anti-CTGF antibody (ab5097)

    ab5097 staining CTGF in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5097 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-CTGF antibody (ab5097)
    Western blot - Anti-CTGF antibody (ab5097)

    Western blot, on recombinant Human CTGF produced in E. coli, using ab5097 at 1/500.

    Not yet tested against endogenous CTGF

     Secondary antibody - anti-rabbit HRP (ab6721)

  • Immunocytochemistry - Anti-CTGF antibody (ab5097)
    Immunocytochemistry - Anti-CTGF antibody (ab5097)This image is courtesy of an Abreview submitted by Beate Thode

    ab5097 staining CTGF in Human Hela, cervix cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 1% normal goat serum for 30 minutes. Samples were incubated with primary antibody (1/750) for 4 hours at 20°C. A Cy3®-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody (1/800).

    See Abreview

参考文献 (23)

ab5097 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5097 は 23 報の論文で使用されています。

  • Dong L  et al. Long non-coding RNA PVT1 promotes the proliferation, migration and EMT process of ovarian cancer cells by regulating CTGF. Oncol Lett 25:71 (2023). PubMed: 36688109
  • Bhat IP  et al. Connective tissue growth factor expression hints at aggressive nature of colorectal cancer. World J Gastroenterol 28:547-569 (2022). PubMed: 35316957
  • Hoffman DB  et al. Temporal changes in the muscle extracellular matrix due to volumetric muscle loss injury. Connect Tissue Res 63:124-137 (2022). PubMed: 33535825
  • Jang SD  et al. Anti-Cancer Activity Profiling of Chemotherapeutic Agents in 3D Co-Cultures of Pancreatic Tumor Spheroids with Cancer-Associated Fibroblasts and Macrophages. Cancers (Basel) 13:N/A (2021). PubMed: 34885065
  • Verhagen JMA  et al. Multi-Omics Profiling in Marfan Syndrome: Further Insights into the Molecular Mechanisms Involved in Aortic Disease. Int J Mol Sci 23:N/A (2021). PubMed: 35008861
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 27 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9.0
Permeabilization
No
Specification
pancreas
Blocking step
SuperBlock™ Blocking Buffer Thermofisher as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 23 2024

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CTGF antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 10 2016

Immunohistochemistry (Frozen sections) abreview for Anti-CTGF antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Ureter)
Specification
Ureter
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 % Trition/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Beate Thode

Verified customer

投稿 Feb 22 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-CTGF antibody

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Hela, Cervix Cancer Cells)
Specification
Hela, Cervix Cancer Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0,2 Triton/PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Beate Thode

Verified customer

投稿 Feb 22 2013

Question

Thank you for your email and for the kind offer. I choose the replacement for another antibody: anti-Fibronectin [IST-9] ab6328.
Many thanks
Kind regards

Read More
Answer

Thank you for your reply and for your cooperation.

As agreed, I have issued a free of charge replacement with one unit of ab6328; the order number is ****.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Question

Merci de votre email et de me tenir au courant des resultats des tests entrepris.

C’est dommage que l’anticorps ne marche pas, mais confirme les resultats que j’avais obtenus.

La difficulte a detecter la protein endogene est peut etre liee au fait que le facteur soir secrete par les cellules, du coup elle ne serait pas tres enrichie dans des lysats cellulaires… n’ayant pas suffisament de temps pour retester de nouveaux anticorps, j’ai abandone l’otption WB pour le CTGF au final.

Merci

Cordialement

Read More
Answer

En compensation de tous les problèmes rencontrés avec les anti-CTGF, j'aimerais vous offrir un avoir contre la commande originale du ab5097 ou un anticorps de remplacement de votre choix.

Merci de m'indiquer la résolution qui vous conviendrait le mieux.

Read More

Question

I finally managed to find some time to run a few more tests with the new antibodies against CTGF you sent me a few weeks ago (see power point file attached).

I used human dermal fibroblast cells that were stimulated or not by 10ng/ml TGFb1 for 24h as a positive control (CTGF is well known to be strongly increased by TGFb1 treatment)

I have also used two other protein ladders from different suppliers in parallel of my usual one to compare MW estimation and they gave the same result.



The new lot (GR42950-1) of the reference ab5097 gave exactly the same results than the previous (GR75927): a single strong band around 50-55kDa that is not varying with TGFb1 treatment.



The other reference ab6992 gives a different pattern.

I initially tried 1/5000 dilution as advised in the datasheet but the signal was weak. With longer exposure (and consequently a very strong background) the same band at 55kDa appears, so as two bands around 40 and 45kDa.

The second test with more protein loaded and lower antibody dilution (1/1000) exhibited the same pattern, bands at 55kDa and 45kDa not varying much upon TGFb1 treatment. But interestingly, there was a diffuse band around 40kDa only in the TGFb1 stimulated sample, bjut absent in the control lysate, which I guess is the specific one for CTGF.

However, you can see the WB stills displays high background (5% BSA blocking though), and the non specific band appears as strong, if not stronger, than the specific one. These non specific bands are present in the pig lysate (last line) whereas the 40kDa band is (I thought I had a positive staining in IHC-Fr for CTGF on pig skin frozen sections with ab5097, the epidermis was strongly positive but I also saw a diffuse positive staining in the healing wound bed in the dermis, where I was expecting the signal to be, but now I have doubts that ab5097 may not be specific in IHC-Fr either).

Are there other antibodies working on pig for IHC-Fr I could try to check?



I don’t know what else to change to get a cleaner blot on human lysates with ab6992. Would you have other advices? Could you let me know what were the exact conditions used to validate this ab6992 please? (especially transfer on nitrocellulose or PVDF?)



Thanks

Best regards

Read More
Answer

J'espère que vous allez bien.

J'aimerais vous informer que mes collègues du laboratoire ont effectué de nouveaux tests en Western Blot avec l'anti-CTGF référence ab5097.

Ils ont essayé différents lysats tissulaires et cellulaire, y compris celui que vous m'aviez suggéré - NIH 3T3 (Mouse embryonic fibroblast cell line) Whole cell lysate, TGF beta 1 treated. Malgré les différentes optimisations, il n'a pas été possible d'obtenir un bon résultat en Western Blot avec la protéine endogène, mais uniquement avec la protéine recombinante. La fiche technique a d'ailleurs été mise à jour avec cette information.

J'espère que ces informations vous seront utiles. N'hésitez pas à me contacter de nouveau si vous avez des questions ou commentaires au sujet de cet anticorps.


Monoclonal de lapin gratuit pour tout achat d'anticorps primaire, dans la limite des stocks disponibles. Notez le code "RABMAB-XBSMG" sur votre prochaine commande d’anticorps primaire. Pour plus d'informations, visitez le lien suivant: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Question

I would like to know the residues / sequence of your CTGF peptide (ab13741).

In other words: Which residues exactly were used to generae Anti-CTGF antibody (ab5097) ?

Read More
Answer

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

Should you have any further questions, please do not hesitate to contact us.

Read More

Question

Dear Kate,

I have just submitted my second abreview for the testing offer agreed with you a few months ago, see below.

I was wondering if I had to order the free antibodies before the 20th of august, or if this expiry date referred only to the limit date to send an abreview feedback.

Many thanks

Best regards

Read More
Answer

Thank you for your message. We appreciate your valuable feedback in the Abreviews.

I can confirm that you will need to place your order using the testing discount code forthe free of charge antibody before 20th August.

I hope this information will be helpful. If you have any further questions, please do not hesitate to contact me.

Read More

Question

As discussed over the phone, here are the reports about the different tests I’ve performed with anti CTGF antibodies ab5097 and ab6992 on pig skin frozen sections (IHC-Fr).

The panel for CTGF 5097 on wounded skin would be the one to add to the abreview, but I’m not sure the staining will be visible as it’s not very bright. It shows some positive staining in the dermis of the wound bed, whereas the dermis of unwounded skin stays negative.

The 2nd page is IHC-Fr on the same sections with ab6992,( but a slightly different protocol, so that’s difficult to compare directly, sorry!). This aniobody wasn’t supposed to work on pig, but I still tried it… there is still a very strong staining in the epidermis (?) but nothing in the wounded dermis where I expected to detect some expression like with ab5097…

Read More
Answer

Merci beaucoup pour ce complément d'information.

Je vous contacterai de nouveau dès que j'aurai reçu les résultats du labo pour les anti-CTGF.

Je vous souhaite un très bon week-end.

Read More

1-10 of 27 Abreviews or Q&A

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