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リコンビナントRabMAb

Anti-CT A 抗体 [EPR3940(2)] (ab109263)

Submit a review Submit a question References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
  • Anti-CT A antibody [EPR3940(2)] (ab109263)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3940(2)] to CT A
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CT A antibody [EPR3940(2)]
    CT A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3940(2)] to CT A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HepG2, A549, K562 , Mouse brain, and RAW 264.7 lysates. IHC-P: human colon, human prostatic hyperplasia tissue and mouse pancreas tissues. ICC/IF: HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109263の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1:500 in human tissues / 1:1000 in mouse tissues

See IHC antigen retrieval protocols.
ICC/IF
1/50.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

1:500 in human tissues / 1:1000 in mouse tissues

See IHC antigen retrieval protocols.
ICC/IF
1/50.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    Controls phosphatidylcholine synthesis.

  • パスウェイ

    Phospholipid metabolism; phosphatidylcholine biosynthesis; phosphatidylcholine from phosphocholine: step 1/2.

  • 配列類似性

    Belongs to the cytidylyltransferase family.

  • 翻訳後修飾

    The serine residues of the C-terminus are phosphorylated. The inactive soluble form is stabilized by phosphorylation, the active membrane bound form is promoted by anionic lipids or diacylglycerol, and is stabilized by dephosphorylation.

  • 細胞内局在

    Cytoplasm > cytosol. Membrane. It can interconvert between an inactive cytosolic form and an active membrane-bound form.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CCT A antibody
      • CCT alpha antibody
      • CCT-alpha antibody
      • Choline phosphate cytidylyltransferase A antibody
      • Choline-phosphate cytidylyltransferase A antibody
      • CT A antibody
      • CT antibody
      • CTP:phosphocholine cytidylyltransferase A antibody
      • CTPCT antibody
      • PCY1A_HUMAN antibody
      • PCYT1 antibody
      • Pcyt1a antibody
      • Phosphate cytidylyltransferase 1 choline alpha antibody
      • Phosphate cytidylyltransferase 1 choline alpha isoform antibody
      • Phosphorylcholine transferase A antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue sections labeling CT A with Purified ab109263 at 1:1000 dilution (0.53 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunocytochemistry/ Immunofluorescence - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CT A with Purified ab109263 at 1:50 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CT A with Purified ab109263 at 1:500 dilution (1.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified) + RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/5000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse brain lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Western blot - Anti-CT A antibody [EPR3940(2)] (ab109263)
      All lanes : Anti-CT A antibody [EPR3940(2)] (ab109263) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 42 kDa
      Observed band size: 42 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

      ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human colon tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CT A antibody [EPR3940(2)] (ab109263)

      ab109263, at a 1/50 dilution, staining CT A in paraffin embedded Human prostatic hyperplasia by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-CT A antibody [EPR3940(2)] (ab109263)
      Anti-CT A antibody [EPR3940(2)] (ab109263)

    参考文献 (9)

    ab109263 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109263 は 9 報の論文で使用されています。

    • Östlund C  et al. Hepatocytes Deficient in Nuclear Envelope Protein Lamina-associated Polypeptide 1 are an Ideal Mammalian System to Study Intranuclear Lipid Droplets. J Lipid Res 63:100277 (2022). PubMed: 36100089
    • Christensen KE  et al. Mild Choline Deficiency and MTHFD1 Synthetase Deficiency Interact to Increase Incidence of Developmental Delays and Defects in Mice. Nutrients 14:N/A (2021). PubMed: 35011003
    • Yang J  et al. CCT a is a novel biomarker for diagnosis of laryngeal squamous cell cancer. Sci Rep 9:11823 (2019). PubMed: 31413263
    • Petkevicius K  et al. Accelerated phosphatidylcholine turnover in macrophages promotes adipose tissue inflammation in obesity. Elife 8:N/A (2019). PubMed: 31418690
    • Huang Q  et al. Stimulated phospholipid synthesis is key for hepatitis B virus replications. Sci Rep 9:12989 (2019). PubMed: 31506451
    • Chen S  et al. In vitro fertilization alters phospholipid profiles in mouse placenta. J Assist Reprod Genet N/A:N/A (2019). PubMed: 30610659
    • Viswanath P  et al. 2-Hydroxyglutarate-Mediated Autophagy of the Endoplasmic Reticulum Leads to an Unusual Downregulation of Phospholipid Biosynthesis in Mutant IDH1 Gliomas. Cancer Res 78:2290-2304 (2018). PubMed: 29358170
    • Haider A  et al. PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress. Dev Cell 45:481-495.e8 (2018). PubMed: 29754800
    • Payne F  et al. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A 111:8901-6 (2014). WB ; Human . PubMed: 24889630

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