Anti-CstF-50 抗体 [EPR12332] (ab173288)
![Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.co.jp/ps/products/173/ab173288/Images/ab173288-196670-ab173288WB1.jpg "Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12332] to CstF-50
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CstF-50 antibody [EPR12332]
CstF-50 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12332] to CstF-50 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment within Human CstF-50. The exact sequence is proprietary.
Database link: Q05048 -
ポジティブ･コントロール
- 293T, HeLa, A549 and K562 cell lysates; Human cervical carcinoma and testis tissues; A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12332 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50 - 1/100.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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| 特記事項 |
|---|
|
ICC/IF
1/50 - 1/100. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
ターゲット情報
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機能
One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA. -
配列類似性
Contains 6 WD repeats. -
ドメイン
The WD6 domain is required for interaction with BARD1. WD domains are responsible for interaction with CSTF3.
N-terminus mediates homodimerization. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1477 Human
- Entrez Gene: 67337 Mouse
- Entrez Gene: 311670 Rat
- Omim: 600369 Human
- SwissProt: Q05048 Human
- SwissProt: Q99LC2 Mouse
- SwissProt: Q5BJQ6 Rat
- Unigene: 172865 Human
see all -
別名
- CF 1 50 kDa subunit antibody
- CF-1 50 kDa subunit antibody
- Cleavage stimulation factor 50 kDa subunit antibody
see all
画像
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Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)All lanes : Anti-CstF-50 antibody [EPR12332] (ab173288) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 48 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.co.jp/ps/products/173/ab173288/Images/ab173288-196671-ab173288IHC11.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CstF-50 with ab173288 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.co.jp/ps/products/173/ab173288/Images/ab173288-196672-ab173288IHC21.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CstF-50 with ab173288 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.co.jp/ps/products/173/ab173288/Images/ab173288-196673-ab173288ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CstF-50 antibody [EPR12332] (ab173288)Immunofluorescent analysis of A549 cells labeling CstF-50 with ab173288 at 1/50 dilution.
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![Anti-CstF-50 antibody [EPR12332] (ab173288)](https://www.abcam.co.jp/ps/products/173/ab173288/Images/ab173288-5-benefits-of-recombinant-antibodies.png)
Anti-CstF-50 antibody [EPR12332] (ab173288)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab173288 は論文での使用が確認できていません。