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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CSNK2A1 抗体 [EP1963Y] (ab76040)

Submit a review Q&A (1)References (11)
Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
  • Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1963Y] to CSNK2A1
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

製品の概要

  • 製品名

    Anti-CSNK2A1 antibody [EP1963Y]
    CSNK2A1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP1963Y] to CSNK2A1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa and HCT116 cell lysate, Mouse and Rat brain lysate. Flow cyto(intra): Jurkat (Human T cell leukemia T lymphocyte) cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76040の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
特記事項
Flow Cyt (Intra)
1/20 - 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
追加情報
Is unsuitable for ICC/IF or IP.

ターゲット情報

  • 機能

    Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.

  • 配列類似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Casein kinase 2 alpha 1 polypeptide antibody
    • Casein kinase 2 alpha antibody
    • Casein kinase II alpha 1 antibody
    • Casein kinase II alpha 1 subunit antibody
    • Casein kinase II alpha subunit antibody
    • Casein kinase II subunit alpha antibody
    • CK II alpha antibody
    • CK II antibody
    • CK2 alpha antibody
    • CK2 catalytic subunit alpha antibody
    • CK2A1 antibody
    • CKII antibody
    • CKIIalpha antibody
    • CSK21_HUMAN antibody
    • CSNK2A1 antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Flow Cytometry (Intracellular) - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CSNK2A1 with purified ab76040 at 1:20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brian lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/1000 dilution

    Lane 1 : Wild-type HCT116 cell lysate
    Lane 2 : CSNK2A1 knockout HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab76040 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab76040 Anti-CSNK2A1 antibody [EP1963Y] was shown to specifically react with CSNK2A1 in wild-type HCT116 cells. Loss of signal was observed when knockout cell line ab266864 (knockout cell lysate ab257401) was used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Western blot - Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    All lanes : Anti-CSNK2A1 antibody [EP1963Y] (ab76040) at 1/500 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : CSNK2A1 knockout HAP1 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 45 kDa



    Lanes 1 - 4: Merged signal (red and green). Green – ab76040 observed at 45 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76040 was shown to specifically react with CSNK2A1 when CCSNK2A1 knockout samples were used. Wild-type and CSNK2A1 knockout samples were subjected to SDS-PAGE. ab76040 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Anti-CSNK2A1 antibody [EP1963Y] (ab76040)
    Anti-CSNK2A1 antibody [EP1963Y] (ab76040)

参考文献 (11)

ab76040 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76040 は 11 報の論文で使用されています。

  • Wang C  et al. CDK11 requires a critical activator SAP30BP to regulate pre-mRNA splicing. EMBO J 42:e114051 (2023). PubMed: 38059508
  • Sun Z  et al. Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Front Cell Neurosci 16:911973 (2022). PubMed: 35928572
  • Yang W  et al. Protein Kinase CK2 Controls CD8+ T Cell Effector and Memory Function during Infection. J Immunol 209:896-906 (2022). PubMed: 35914835
  • Sato K  et al. Simultaneous CK2/TNIK/DYRK1 inhibition by 108600 suppresses triple negative breast cancer stem cells and chemotherapy-resistant disease. Nat Commun 12:4671 (2021). PubMed: 34344863
  • Trembley JH  et al. Identification of high protein kinase CK2α in HPV(+) oropharyngeal squamous cell carcinoma and correlation with clinical outcomes. PeerJ 9:e12519 (2021). PubMed: 34993017
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Question

Does this antibody cross-react with CK II alpha'?

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Answer

Thank you for your phone call and for your patience while I have been in touch with the lab about ab76040. The immunogen of the antibody shares 64% identity with murine CK II alpha prime protein, so cross-reactivity is not predicted. However, the cross-reactivity has not been tested so we can not guarantee this. If you have any further questions or would like to align the immunogens of other antibodies to CK II alpha prime, please do let me know.

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