Anti-CSN4 抗体 (ab12322)
Key features and details
- Rabbit polyclonal to CSN4
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CSN4 antibody
CSN4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CSN4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide within Human CSN4 aa 350 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9BT78 -
ポジティブ･コントロール
- WB: HeLa, HEK293T, Jurkat, TCMK-1, NIH/3T3
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12322の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 49 kDa.
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| 特記事項 |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 49 kDa. |
ターゲット情報
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機能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
配列類似性
Belongs to the CSN4 family.
Contains 1 PCI domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 422594 Chicken
- Entrez Gene: 461207 Chimpanzee
- Entrez Gene: 540223 Cow
- Entrez Gene: 51138 Human
- Entrez Gene: 26891 Mouse
- Entrez Gene: 100125960 Pig
- Entrez Gene: 360915 Rat
- Entrez Gene: 693435 Rhesus monkey
see all -
別名
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
see all
画像
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Western blot - Anti-CSN4 antibody (ab12322)All lanes : Anti-CSN4 antibody (ab12322) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 49 kDa
Exposure time: 3 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab12322 は 4 報の論文で使用されています。
- Chung D et al. Inhibition of neddylation induces mitotic defects and alters MKLP1 accumulation at the midbody during cytokinesis. Cell Cycle 18:1135-1153 (2019). PubMed: 31057046
- Füzesi-Levi MG et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . PubMed: 24421388
- Bhansali M & Shemshedini L COP9 subunits 4 and 5 target soluble guanylyl cyclase a1 and p53 in prostate cancer cells. Mol Endocrinol 28:834-45 (2014). PubMed: 24725084
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822