Anti-CSN1 抗体 (ab10413)
Key features and details
- Rabbit polyclonal to CSN1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CSN1 antibody
CSN1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CSN1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide corresponding to Human CSN1 aa 400 to the C-terminus (C terminal).
Database link: 2873 -
ポジティブ･コントロール
- Whole cell lysate from mock transfected or CSN1 transfected HEK293T cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa.
|
|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa. |
|
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein-and mitogen-activated protein kinase-mediated signal transduction. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the CSN1 family.
Contains 1 PCI domain. -
ドメイン
The PCI domain is necessary and sufficient for the interactions with other CSN subunits of the complex. Mediates the interaction with CAPN8.
The N-terminal part (1-216), which is not required for deneddylating activity and CSN complex formation, is nevertheless essential for other aspects of CSN complex function, such as repression of c-fos/FOS expression. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 417382 Chicken
- Entrez Gene: 747173 Chimpanzee
- Entrez Gene: 525572 Cow
- Entrez Gene: 2873 Human
- Entrez Gene: 209318 Mouse
- Entrez Gene: 117039 Rat
- Omim: 601934 Human
- SwissProt: Q13098 Human
see all -
別名
- COP9 signalosome complex subunit 1 antibody
- COP9 signalosome subunit 1 antibody
- COPS1 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN1 antibody (ab10413)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CSN1 with ab10413 at 1/1000 (1µg/ml). Detection: DAB.
-
Western blot - Anti-CSN1 antibody (ab10413)Detection of Human CSN1 by Western Blot. Samples: Whole cell lysate (50 mcg – E; 10 mcg – T) from mock transfected (E) or CSN1 transfected (T) HEK293T cells.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab10413 は 6 報の論文で使用されています。
- Diviccaro S et al. Neuroactive Steroid-Gut Microbiota Interaction in T2DM Diabetic Encephalopathy. Biomolecules 13:N/A (2023). PubMed: 37759725
- Valencia AP et al. Aging Increases Susceptibility to Develop Cardiac Hypertrophy following High Sugar Consumption. Nutrients 14:N/A (2022). PubMed: 36364920
- Nara K et al. Anti-inflammatory roles of microRNA 21 in lipopolysaccharide-stimulated human dental pulp cells. J Cell Physiol N/A:N/A (2019). PubMed: 31042008
- Gremminger VL et al. Compromised Exercise Capacity and Mitochondrial Dysfunction in the Osteogenesis Imperfecta Murine (oim) Mouse Model. J Bone Miner Res 34:1646-1659 (2019). PubMed: 30908713
- Pesaresi M et al. Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features. Biol Sex Differ 9:6 (2018). PubMed: 29351809
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822