Anti-CSK 抗体 - C-terminal (ab186069)

Western blot: Anti-CSK antibody (ab186069) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab186069 was shown to bind specifically to CSK. A band was observed at 50 kDa in wild-type A549 cell lysates with no signal observed at this size in CSK knockout cell line. To generate this image, wild-type and CSK knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

Immunoprecipitation of CSK from Jurkat whole cell lysate by ab186069 at 1 µg/mg lysate (lane 1). 1 mg of Jurkat whole cell lysate was used, 20% of immunoprecipitate was loaded. Lane 2: IgG control. Detection: chemiluminescence. Exposure time: 10 seconds.