Anti-CSK 抗体 (ab744)
Key features and details
- Goat polyclonal to CSK
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CSK antibody
CSK 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CSK -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Dog
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免疫原
Synthetic peptide corresponding to Human CSK aa 400 to the C-terminus (C terminal).
Database link: NP_004374.1 -
ポジティブ･コントロール
- Jurkat cell lysate, Human colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab744の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. -
組織特異性
Expressed in lung and macrophages. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylation of Tyr-304 occurs only at abnormally high CSK concentrations in vitro. -
細胞内局在
Cytoplasm. Cell membrane. Mainly cytoplasmic, also present in lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 509246 Cow
- Entrez Gene: 487649 Dog
- Entrez Gene: 1445 Human
- Entrez Gene: 12988 Mouse
- Omim: 124095 Human
- SwissProt: Q0VBZ0 Cow
- SwissProt: P41240 Human
- SwissProt: P41241 Mouse
see all -
別名
- C SRC antibody
- C SRC kinase antibody
- C src Tyrosine Kinase antibody
see all
画像
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Western blot - Anti-CSK antibody (ab744)ab744 staining (0.5
µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab744 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSK antibody (ab744)ab744 at 4µg/ml staining CSK in Human Colon by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. -
Western blot - Anti-CSK antibody (ab744)Anti-CSK antibody (ab744) at 1 µg/ml + Mouse spleen lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab744 は 2 報の論文で使用されています。
- Yeh WL et al. Fulvestrant-induced cell death and proteasomal degradation of estrogen receptor a protein in MCF-7 cells require the CSK c-Src tyrosine kinase. PLoS One 8:e60889 (2013). WB ; Human . PubMed: 23593342
- Ribeiro-Oliveira A et al. Protein western array analysis in human pituitary tumours: insights and limitations. Endocr Relat Cancer 15:1099-114 (2008). WB ; Human . PubMed: 18701577