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リコンビナントRabMAb

Anti-CRTH2 抗体 [EPR25167-36] - BSA and Azide free (ab302921)

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Western blot - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
  • Immunoprecipitation - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
  • Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25167-36] to CRTH2 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-CRTH2 antibody [EPR25167-36] - BSA and Azide free
    CRTH2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25167-36] to CRTH2 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF,IHC-Fr or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human, rat and mouse cerebellum whole tissue lysate. IP: Human cerebellum tissue lysate.

  • 特記事項

    AB302921 is the carrier-free version of AB302920.

    AB302920 does not react with human in IHC, ICC/IF or Flow Cytometry (Intra); mouse in IHC, IHC-Fr or IP; rat in IHC or IHC-Fr.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Constituent: 100% PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR25167-36

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302921の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 43,55 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 43,55 kDa (predicted molecular weight: 43 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF,IHC-Fr or IHC-P.

ターゲット情報

  • 機能

    Orphan receptor.

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • CD294 antibody
    • CD294 antigen antibody
    • Chemoattractant receptor homologous molecule expressed on Th2 cells antibody
    • chemoattractant receptor-homologous antibody
    • Chemoattractant receptor-homologous molecule expressed on Th2 cells antibody
    • CRTH 2 antibody
    • CRTH2 antibody
    • DL1R antibody
    • DP2 prostaglandin receptor antibody
    • G Protein Coupled Receptor 44 antibody
    • GPR 44 antibody
    • Gpr44 antibody
    • GPR44_HUMAN antibody
    • PTGDR2 antibody
    • Putative G protein coupled receptor 44 antibody
    • Putative G-protein coupled receptor 44 antibody
    see all

画像

  • Western blot - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
    Western blot - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
    All lanes : Anti-CRTH2 antibody [EPR25167-36] (ab302920) at 1/1000 dilution

    Lane 1 : Human cerebellum tissue lysate
    Lane 2 : Human heart tissue lysate
    Lane 3 : Rat cerebellum tissue lysate
    Lane 4 : Rat heart tissue lysate
    Lane 5 : Mouse cerebellum tissue lysate
    Lane 6 : Mouse heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 43 kDa
    Observed band size: 43,55 kDa why is the actual band size different from the predicted?



    This data was developed using AB302920, the same antibody clone in a different buffer formulation.

    Blocking / Diluting buffer: 5% NFDM/TBST.

    Exposure times:

    Lane 1-2: 48 seconds
    Lane 3-6: 1 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 24929001).
    Negative control: heart (PMID: 27103662).

  • Immunoprecipitation - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
    Immunoprecipitation - Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)

    This data was developed using AB302920, the same antibody clone in a different buffer formulation.

    CRTH2 was immunoprecipitated from 0.35 mg human cerebellum tissue lysate 10 µg with ab302920 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab302920 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: human cerebellum tissue lysate 10 µg

    Lane 2: ab302920 IP in human cerebellum tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302920 in human cerebellum tissue lysate

    Blocking and dilution buffer: 5% NFDM/TBST.

    Observed MW (kDa): 43,55

    Exposure time: 15 seconds.

  • Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)
    Anti-CRTH2 antibody [EPR25167-36] (BSA and Azide free) (AB302921)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab302921 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302921 は論文での使用が確認できていません。

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