Anti-CROT/COT 抗体 (ab175450)
Key features and details
- Goat polyclonal to CROT/COT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-CROT/COT antibody
CROT/COT 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CROT/COT -
由来種
Goat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Dog
-
免疫原
Synthetic peptide corresponding to Rat CROT/COT aa 178-199 (internal sequence). NP_114193.1.
Sequence:NYFKTESEGHCPTH
Database link: P11466 -
ポジティブ･コントロール
- Mouse liver lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab175450 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175450の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 70 kDa.
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 70 kDa. 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
機能
Beta-oxidation of fatty acids. The highest activity concerns the C6 to C10 chain length substrate. Converts the end product of pristanic acid beta oxidation, 4,8-dimethylnonanoyl-CoA, to its corresponding carnitine ester. -
パスウェイ
Lipid metabolism; fatty acid beta-oxidation. -
配列類似性
Belongs to the carnitine/choline acetyltransferase family. -
細胞内局在
Peroxisome. - Information by UniProt
-
参照データベース
- Entrez Gene: 482283 Dog
- Entrez Gene: 54677 Human
- Entrez Gene: 74114 Mouse
- Entrez Gene: 83842 Rat
- Omim: 606090 Human
- SwissProt: Q9UKG9 Human
- SwissProt: Q9DC50 Mouse
- SwissProt: P11466 Rat
see all -
別名
- Carnitine O octanoyltransferase antibody
- COT antibody
- CROT antibody
see all
画像
-
Western blot - Anti-CROT/COT antibody (ab175450)Anti-CROT/COT antibody (ab175450) at 1 µg/ml + mouse liver lysates at 35 µg
Predicted band size: 70 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
-
Datasheet download
参考文献 (2)
ab175450 は 2 報の論文で使用されています。
- Wang JL et al. Mesenchymal stem cells ameliorate lipid metabolism through reducing mitochondrial damage of hepatocytes in the treatment of post-hepatectomy liver failure. Cell Death Dis 12:111 (2021). PubMed: 33479191
- Ni Y et al. LncRNA EGOT regulates the proliferation and apoptosis of colorectal cancer by miR-33b-5p/CROT axis. Biosci Rep N/A:N/A (2020). PubMed: 32373939
