Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-CRMP3 抗体 (ab36217)

Submit a review Q&A (5)References (3)
Western blot - Anti-CRMP3 antibody (ab36217)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP3 antibody (ab36217)

Key features and details

  • Rabbit polyclonal to CRMP3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CRMP3 antibody
    CRMP3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CRMP3

  • 由来種

    Rabbit

  • 特異性

    No cross reactivity was detected with CRMP1, CRMP2, CRMP4 or CRMP5.

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    A 15 residue synthetic peptide derived from C-terminal domain of human CRMP3.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab36217の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Predicted molecular weight: 65 kDa.
IHC-P
Use at an assay dependent concentration.
特記事項
WB
1/2000. Predicted molecular weight: 65 kDa.
IHC-P
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

  • 配列類似性

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Collapsin response mediator protein 3 antibody
      • CRMP 3 antibody
      • CRMP-3 antibody
      • CRMP3 antibody
      • Dihydropyrimidinase like 4 antibody
      • Dihydropyrimidinase related protein 4 antibody
      • Dihydropyrimidinase-related protein 4 antibody
      • DPYL4_HUMAN antibody
      • DPYSL4 antibody
      • DRP 4 antibody
      • DRP-4 antibody
      • DRP4 antibody
      • ULIP 4 antibody
      • ULIP-4 antibody
      • ULIP4 antibody
      • UNC33 like phosphoprotein 4 antibody
      • UNC33-like phosphoprotein 4 antibody
      see all

    画像

    • Western blot - Anti-CRMP3 antibody (ab36217)
      Western blot - Anti-CRMP3 antibody (ab36217)
      All lanes : Anti-CRMP3 antibody (ab36217)

      Lane 1 : E.coli transformed with cDNA of human CRMP1
      Lane 2 : E.coli transformed with cDNA of human CRMP2
      Lane 3 : E.coli transformed with cDNA of human CRMP3
      Lane 4 : E.coli transformed with cDNA of human CRMP4
      Lane 5 : E.coli transformed with cDNA of human CRMP5

      Predicted band size: 65 kDa
      Observed band size: 65 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP3 antibody (ab36217)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP3 antibody (ab36217)
      IHC image of ab36217 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36217, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献 (3)

    ab36217 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab36217 は 3 報の論文で使用されています。

    • Lin YF  et al. Elevated Hippocampal CRMP5 Mediates Chronic Stress-Induced Cognitive Deficits by Disrupting Synaptic Plasticity, Hindering AMPAR Trafficking, and Triggering Cytokine Release. Int J Mol Sci 24:N/A (2023). PubMed: 36902337
    • Lin YS  et al. Collapsin response mediator protein 5 (CRMP5) causes social deficits and accelerates memory loss in an animal model of Alzheimer's disease. Neuropharmacology 157:107673 (2019). PubMed: 31233825
    • Hou ST  et al. Collapsin response mediator protein 3 deacetylates histone H4 to mediate nuclear condensation and neuronal death. Sci Rep 3:1350 (2013). ChIP ; Mouse . PubMed: 23443259

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    Dear,
    With the use of the troubleshooting tips you provided us with, we were able to achieve a good result for Western blot for ab36217.
    Many thanks for your help and service,
    Kind regards,

    Read More
    Answer

    Thank you very much for your reply.

    I am very happy to hear the troubleshooting tips were useful.

    Please do not hesitate to contact us back for further assistance. Have a very nice day.

    Read More

    Question

    Dear,

    In attachment you can find our answers to the questionnaire.

    Many thanks in advance,

    Best regards,

    Read More
    Answer

    Thank you for taking the time to complete our questionnaire and sending the image. Reviewing this case, I would like to offer some suggestions to help optimise the results from ab36217.

    - As a lysis buffer I would suggest using RIPA (radio immuno precipitation assay) buffer due to its higher denaturing level. You can prepare it following the recipe under the next link:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11414

    - It could be the membranes are blocked in excess. I would recommend diluting both, the primary and secondary antibody in TBS without milk. You could even try using 5% BSA as a blocking agent instead of milk, which is a milder blocking agent.

    I hope these suggestions will help to improve your results. If our troubleshooting tips do not help, please let us know how you would like to proceed and I would be happy to assist you further in resolving the problem you are experiencing.

    Read More

    Question

    Dear,I purchased the CRMP-3 antibody (see previous mail), and tested this with Western blotting on mouse cerebellar extract samples. However, this failed to give a good result after trying a series of different dilutions and conditions.
    As such, I was wondering, since you guaranteed a replacement in case that this antibody would not work, if it is possible to ask for another Abcam antibody?Kind regards,

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear you are experiencing difficulties with our products.

    It is very difficult for us to understand the problem without further details. In order to monitor the product’s quality, and better understand the problem, I would appreciate if you could please send us the attached questionnaire completed. It only takes about 5 minutes to fill out; any images would also be very helpful.

    I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Dear,
    Thank you very much for your kind reply.
    However, because there are no references for this antibody yet, and thus there is no 100% guarantee that it will work properly, I was wondering whether it would be possible to receive a small sample to test for this antibody, whereafter we would purchase the antibody if it gives a good result.
    Kind regards,

    Read More
    Answer

    Thank you for your reply.

    Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of ab36217.

    If there is any concrete question you need to know about the specificity of this antibody I will be more than happy to help you.

    In any case, all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Dear,
    On your website, we found the Anti-CRMP3 antibody ab36217, which seems a very interesting one for us to purchase. However, we would like to get more information about performed experiments from other research groups with this antibody.
    As I did not find any references on your website, I wondered if this is still the case. Otherwise, we would be very pleased with additional information/references concerning this antibody.
    Kind regards and many thanks in advance,

    Read More
    Answer

    Thank you for contacting us.

    All of the information and images we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention. Unfortunately, we do not have any references or publications available for this particular antibody.

    However, this antibody is guaranteed to work in with WB and IHC (in frozen and paraffin embedded sections). If you had any problems using such applications, we would them send you a replacement or reimbursement as guaranteed by the Abpromise guarantee.

    I am very sorry I couldn’t help you further. Please do not hesitate to contact us if you need any other advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com