Anti-CRMP2 抗体 (ab36201)
Key features and details
- Rabbit polyclonal to CRMP2
- Suitable for: WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CRMP2 antibody
CRMP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CRMP2 -
由来種
Rabbit -
特異性
No cross reactivity was detected with CRMP1, CRMP3, CRMP4 or CRMP5. -
アプリケーション
適用あり: WB, IHC-Frmore details -
種交差性
交差種: Human -
免疫原
A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab36201の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 63 kDa.
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| IHC-Fr |
1/500.
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| 特記事項 |
|---|
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WB
1/2000. Predicted molecular weight: 63 kDa. |
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IHC-Fr
1/500. |
ターゲット情報
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機能
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily. -
翻訳後修飾
3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 1808 Human
- Omim: 602463 Human
- SwissProt: Q16555 Human
- Unigene: 173381 Human
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別名
- Collapsin response mediator protein 2 antibody
- Collapsin response mediator protein antibody
- Collapsin response mediator protein hCRMP 2 antibody
see all
画像
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Western blot - Anti-CRMP2 antibody (ab36201)All lanes : Anti-CRMP2 antibody (ab36201) at 1/2000 dilution
Lane 1 : Wild-type A549 cell lysate at 20 µg
Lane 2 : DPYSL2 knockout A549 cell lysate at 20 µg
Lane 3 : HeLa cell lysate at 20 µg
Lane 4 : A549 Nuclear Fraction cell lysate at 20 µg
Lane 5 : Recombinant Human CRMP2 protein (ab114269) cell lysate at 0.2 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDaWestern blot: Anti-DPYSL2 antibody (ab36201) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab36201 was shown to bind specifically to DPYSL2. A band was observed at 63 kDa in wild-type A549 cell lysates with no signal observed at this size in DPYSL2 knockout cell line. To generate this image, wild-type and DPYSL2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Western blot - Anti-CRMP2 antibody (ab36201)All lanes : Anti-CRMP2 antibody (ab36201)
Lane 1 : E.coli transformed with cDNA of human CRMP1
Lane 2 : E.coli transformed with cDNA of human CRMP2
Lane 3 : E.coli transformed with cDNA of human CRMP3
Lane 4 : E.coli transformed with cDNA of human CRMP4
Lane 5 : E.coli transformed with cDNA of human CRMP5
Predicted band size: 63 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Frozen sections) - Anti-CRMP2 antibody (ab36201)Ab36201 staining human CRMP2 in olfactory receptor neurons within the olfactory mucosa by immunohistochemistry.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (7)
ab36201 は 7 報の論文で使用されています。
- Simões AP et al. Glutamate-induced and NMDA receptor-mediated neurodegeneration entails P2Y1 receptor activation. Cell Death Dis 9:297 (2018). PubMed: 29463792
- Sukumaran SK et al. Whole transcriptome profiling of taste bud cells. Sci Rep 7:7595 (2017). PubMed: 28790351
- Bajor M et al. Synaptic cell adhesion molecule-2 and collapsin response mediator protein-2 are novel members of the matrix metalloproteinase-9 degradome. J Neurochem 122:775-88 (2012). PubMed: 22694054
- Mileusnic R & Rose SP The memory enhancing effect of the APP-derived tripeptide Ac-rER is mediated through CRMP2. J Neurochem 118:616-25 (2011). PubMed: 21255016
- Samara A et al. Proteomics reveal rat hippocampal lateral asymmetry. Hippocampus 21:108-19 (2011). WB ; Rat . PubMed: 20020437
- Gelman BB & Nguyen TP Synaptic proteins linked to HIV-1 infection and immunoproteasome induction: proteomic analysis of human synaptosomes. J Neuroimmune Pharmacol 5:92-102 (2010). WB ; Human . PubMed: 19693676
- Tumusiime S et al. Regulation of ROCKII by localization to membrane compartments and binding to DynaminI. Biochem Biophys Res Commun 381:393-6 (2009). PubMed: 19222995
