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リコンビナントRabMAb

Anti-CRMP1 抗体 [EPR14520(B)] - N-terminal (ab180517)

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Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
  • Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
  • Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CRMP1 antibody [EPR14520(B)] - N-terminal
    CRMP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human CRMP1 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q14194

  • ポジティブ･コントロール

    • Human cerebellum lysate, Fetal brain lysate, SH-SY5Y cell lysate, Paraffin-embedded Human brain sections.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14520(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180517の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.

  • 組織特異性

    Brain.

  • 配列類似性

    Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.

  • 細胞内局在

    Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes and the mitotic spindle during metaphase.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Collapsin response mediator protein 1 antibody
    • CRMP 1 antibody
    • CRMP-1 antibody
    • Crmp1 antibody
    • Dihydropyrimidinase like 1 antibody
    • Dihydropyrimidinase related protein 1 antibody
    • Dihydropyrimidinase-related protein 1 antibody
    • DPYL1_HUMAN antibody
    • DPYSL1 antibody
    • DRP 1 antibody
    • DRP-1 antibody
    • DRP1 antibody
    • ULIP-3 antibody
    • Ulip3 antibody
    • Unc-33-like phosphoprotein 3 antibody
    see all

画像

  • Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    All lanes : Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/20000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : Fetal brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 62 kDa

  • Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/5000 dilution + Human cerebellum lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 62 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP1 with ab180517 at 1/500 dilution. Anti-rabbit HRP seconday antibody was used and counterstaining with Hematoxylin was performed.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)
    Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

参考文献 (0)

ab180517 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab180517 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

Immunoprecipitation abreview for Anti-CRMP1 antibody [EPR14520(B)] - N-terminal

 Poor
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Squirrel Tissue lysate - other (retina)
Total protein in input
120 µg
Immuno-precipitation step
Protein A
Specification
retina
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Jan 11 2016

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