Anti-Crk p38 抗体 [mAbcam69723] (ab69723)
![Western blot - Anti-Crk p38 antibody [mAbcam69723] (ab69723)](https://www.abcam.co.jp/ps/products/69/ab69723/Images/Crk-p38-Primary-antibodies-ab69723-2.jpg "Western blot - Anti-Crk p38 antibody [mAbcam69723] (ab69723)")
Key features and details
- Mouse monoclonal [mAbcam69723] to Crk p38
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Crk p38 antibody [mAbcam69723]
Crk p38 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam69723] to Crk p38 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: K562; HepG2; HeLa; HEK293; MCF7.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
mAbcam69723 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69723の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 34 kDa). |
ターゲット情報
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機能
The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. -
配列類似性
Belongs to the CRK family.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation.
The SH2 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form.
Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. -
細胞内局在
Cytoplasm. Cell membrane. Translocated to the plasma membrane upon cell adhesion. - Information by UniProt
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参照データベース
- Entrez Gene: 417553 Chicken
- Entrez Gene: 1398 Human
- Entrez Gene: 12928 Mouse
- Entrez Gene: 54245 Rat
- Omim: 164762 Human
- SwissProt: Q04929 Chicken
- SwissProt: P46108 Human
- SwissProt: Q64010 Mouse
see all -
別名
- Adapter molecule crk antibody
- Avian sarcoma virus CT10 (v crk) oncogene homolog antibody
- CRK antibody
see all
画像
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Western blot - Anti-Crk p38 antibody [mAbcam69723] (ab69723)All lanes : Anti-Crk p38 antibody [mAbcam69723] (ab69723) at 1 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 36,45 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Crk p38 contains a number of potential phosphorylation sites (SwissProt) which may explain the migration pattern observed.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab69723 は論文での使用が確認できていません。