Anti-CRISPR-Cas9 抗体

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CRISPR-Cas9 antibody (AB204448)

Flow cytometric analysis of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells (upper panel), and transfected with CRISPR-Cas9 (lower panel) labeling CRISPR-Cas9 with ab204448 at 1/5000 dilution.

Cells were fixed with 1% PFA and incubated with the primary antibody (1/5000) at RT for 2 hours.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CRISPR-Cas9 antibody (AB204448)

Immunofluorescent analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells transfected with DDDDK tag-CRISPR-Cas9, labeling CRISPR-Cas9 with ab204448 at 1/10,000 dilution (green). Labeling with anti-DDDDK tag (red). Right hand panel shows merged image with DAPI staining (blue).

Protocol : Fixation with PFA 2% 10 min, permeabilization cold methanol, block with BSA 2% for 1h at RT, incubation with ab204448 1/10,000 1h 30 min at RT.

There is a low background staining with ab204448 which is minimized at 1/10000 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-CRISPR-Cas9 antibody (AB204448)

Western blot analysis of immunoprecipitate using ab204448 in U-2 OS (Human bone osteosarcoma epithelial cell line) cells extracts.

WB : anti-DDDDK tag.

Lane 1 : Input.
Lane 2 : Immunoprecipitation with control serum (10μl).
Lane 3 : Immunoprecipitation with ab204448 (10μl)

All lanes:

Immunoprecipitation - Anti-CRISPR-Cas9 antibody (ab204448)

false

• WB

Supplier Data

Western blot - Anti-CRISPR-Cas9 antibody (AB204448)

WB : Transfer in nitrocelullose, block : 1 hour 5% milk in TBST (Tween= 0.2%),

Incubation with primary antibody o/n at 4°C in 5% milk TBST (Tween= 0.2%).

Lanes 1 - 2:

Anti-DDDDK tag antibody

Lanes 3 - 4:

Western blot - Anti-CRISPR-Cas9 antibody (ab204448) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 40 µg

Lanes 2 and 4:

HEK-293T cells transfected with DDDDK tag-CRISPR-Cas9 lysate at 40 µg

Lane 3:

HEK-293T cell lysate at 40 µg

Predicted band size: 158 kDa

false