Anti-CRF 抗体 [EPR19154] (ab184238)
![Western blot - Anti-CRF antibody [EPR19154] (ab184238)](https://www.abcam.co.jp/ps/products/184/ab184238/Images/ab184238-253161-anti-corticotropin-releasing-factor-antibody-epr19154-western-blot.jpg "Western blot - Anti-CRF antibody [EPR19154] (ab184238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19154] to CRF
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CRF antibody [EPR19154]
CRF 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR19154] to CRF -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human CRF fragment recombinant protein; Human brain, mouse brain, mouse hypothalamus, rat brain and rat hypothalamus lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR19154 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184238の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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関連性
CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few). -
細胞内局在
Cytoplasmic and Secreted -
参照データベース
- Entrez Gene: 1392 Human
- Entrez Gene: 12918 Mouse
- Entrez Gene: 81648 Rat
- Omim: 122560 Human
- SwissProt: P06850 Human
- SwissProt: Q8CIT0 Mouse
- SwissProt: P01143 Rat
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別名
- Corticoliberin antibody
- Corticotropin releasing factor antibody
- Corticotropin releasing hormone antibody
see all
画像
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Western blot - Anti-CRF antibody [EPR19154] (ab184238)Anti-CRF antibody [EPR19154] (ab184238) at 1/1000 dilution + Human CRF fragment recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human CRF fragment recombinant protein contains aa20-185 with a His-Tag®. This recombinant human fragment protein was made in-house.
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![Western blot - Anti-CRF antibody [EPR19154] (ab184238)](https://www.abcam.co.jp/ps/products/184/ab184238/Images/ab184238-253159-anti-corticotropin-releasing-factor-antibody-epr19154-western-blot.jpg)
Western blot - Anti-CRF antibody [EPR19154] (ab184238)All lanes : Anti-CRF antibody [EPR19154] (ab184238) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hypothalamus tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat hypothalamus tissue lysate
Lane 6 : Mouse pancreas tissue lysate
Lane 7 : Mouse skin tissue lysate
Lane 8 : Rat skin tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-CRF antibody [EPR19154] (ab184238)](https://www.abcam.co.jp/ps/products/184/ab184238/Images/ab184238-3-benefits-of-recombinant-antibodies.png)
Anti-CRF antibody [EPR19154] (ab184238)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (7)
ab184238 は 7 報の論文で使用されています。
- Wang Y et al. Electroacupuncture alleviates perioperative hypothalamus-pituitary-adrenal axis dysfunction via circRNA-miRNA-mRNA networks. Front Mol Neurosci 16:1115569 (2023). PubMed: 36760604
- Guo L et al. Sleep-Disturbance-Induced Microglial Activation Involves CRH-Mediated Galectin 3 and Autophagy Dysregulation. Cells 12:N/A (2022). PubMed: 36611953
- Kavalakatt S et al. Urocortin Neuropeptide Levels Are Impaired in the PBMCs of Overweight Children. Nutrients 14:N/A (2022). PubMed: 35276788
- Wang Y et al. GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity. Front Neurosci 15:703044 (2021). PubMed: 34658758
- Di T et al. Dopaminergic afferents from midbrain to dorsolateral bed nucleus of stria terminalis inhibit release and expression of corticotropin-releasing hormone in paraventricular nucleus. J Neurochem 154:218-234 (2020). PubMed: 32096869
- Bo TB et al. Coprophagy prevention alters microbiome, metabolism, neurochemistry, and cognitive behavior in a small mammal. ISME J 14:2625-2645 (2020). PubMed: 32632263
- Tang Y et al. miR-212 mediates counter-regulation on CRH expression and HPA axis activity in male mice. J Mol Endocrinol 59:365-375 (2017). PubMed: 28912247