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AB184238

Anti-CRF 抗体 [EPR19154]

Anti-CRF antibody [EPR19154]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal CRF antibody. Suitable for WB and reacts with Rat, Mouse, Human, Recombinant full length protein - Human samples. Cited in 7 publications.

別名を表示する

Gm1347, Crh, Corticoliberin, Corticotropin-releasing factor, Corticotropin-releasing hormone, CRF

2 Images
Western blot - Anti-CRF antibody [EPR19154] (AB184238)
  • WB

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (ab184238) at 1/5000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse hypothalamus tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat hypothalamus tissue lysate at 20 µg

Lane 6:

Mouse pancreas tissue lysate at 20 µg

Lane 7:

Mouse skin tissue lysate at 20 µg

Lane 8:

Rat skin tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 25 kDa

false

Exposure time: 3min

Western blot - Anti-CRF antibody [EPR19154] (AB184238)
  • WB

Supplier Data

Western blot - Anti-CRF antibody [EPR19154] (AB184238)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human CRF fragment recombinant protein contains aa20-185 with a His-Tag®. This recombinant human fragment protein was made in-house.

All lanes:

Western blot - Anti-CRF antibody [EPR19154] (ab184238) at 1/1000 dilution

All lanes:

Human CRF fragment recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Exposure time: 1s

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-CRF antibody [EPR19154] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR19154

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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下記製品にもご興味をお持ちいただけるかもしれません:

AB309283

Human CRF ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Corticotropin-releasing factor (CRF) also known as corticotropin-releasing hormone (CRH) is a peptide hormone involved in the stress response. This protein with a mass of approximately 41 kDa is predominantly produced in the hypothalamus and later secreted into the bloodstream. CRF plays an important role in activating the hypothalamic-pituitary-adrenal (HPA) axis stimulating the secretion of adrenocorticotropic hormone (ACTH) from the anterior pituitary gland. Its expression extends beyond the hypothalamus being present in peripheral tissues such as the adrenal glands and gastrointestinal tract.
Biological function summary

Corticotropin-releasing factor functions as a central coordinator of the body's response to stress. CRF engages several receptors including CRF receptors type 1 and 2 which mediate its actions. It forms part of a larger complex involving several other binding proteins that regulate its availability and receptor affinity. By interacting with these receptors CRF modulates diverse physiological processes such as immune responses and energy balance ensuring the maintenance of homeostasis under stress conditions.

Pathways

Corticotropin-releasing factor orchestrates important signaling within the hypothalamic-pituitary-adrenal (HPA) axis and the stress response pathways. CRF is pivotal in the activation of various signaling cascades that influence the production of glucocorticoids like cortisol. Within these pathways CRF interacts closely with proteins such as ACTH which acts downstream of CRF to potentiate adrenal corticosteroid synthesis. Another related cascade involves CRF in neuroendocrine signaling affecting neurotransmitter release and influencing behaviors related to stress and anxiety.

Corticotropin-releasing factor is heavily implicated in depression and anxiety disorders. Alterations in CRF expression and receptor sensitivity have been associated with the pathophysiology of these conditions. For instance abnormal CRF signaling can lead to elevated stress hormone levels contributing to mood disorders. CRF also connects to proteins like the glucocorticoid receptor influencing how the body processes stress-related hormones and impacting the development and progression of stress-related psychiatric disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (PubMed : 23470617).
See full target information Crh
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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 16:1115569 PubMed36760604

2023

Electroacupuncture alleviates perioperative hypothalamus-pituitary-adrenal axis dysfunction circRNA-miRNA-mRNA networks.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Wei Hu,Jing Han,Jiayuan Zheng,Ning Jiang,Yi Feng,Zhanzhuang Tian

Cells 12: PubMed36611953

2022

Sleep-Disturbance-Induced Microglial Activation Involves CRH-Mediated Galectin 3 and Autophagy Dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Liyang Guo,Kirstin M Reed,Ashley Carter,Yan Cheng,Soheil Kazemi Roodsari,Damian Martinez Pineda,Laurie L Wellman,Larry D Sanford,Ming-Lei Guo

Nutrients 14: PubMed35276788

2022

Urocortin Neuropeptide Levels Are Impaired in the PBMCs of Overweight Children.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Kavalakatt,Abdelkrim Khadir,Shihab Kochumon,Dhanya Madhu,Sriraman Devarajan,Maha Hammad,Nada Alam-Eldin,Samia Warsame,Hessa Al-Kandari,Maria AlMahdi,Rasheed Ahmad,Heikki A Koistinen,Jaakko Tuomilehto,Fahd Al-Mulla,Jehad Abubaker,Ali Tiss

Frontiers in neuroscience 15:703044 PubMed34658758

2021

GluN2A/ERK/CREB Signaling Pathway Involved in Electroacupuncture Regulating Hypothalamic-Pituitary-Adrenal Axis Hyperactivity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Jing Han,Jing Zhu,Mizhen Zhang,Minda Ju,Yueshan Du,Zhanzhuang Tian

The ISME journal 14:2625-2645 PubMed32632263

2020

Coprophagy prevention alters microbiome, metabolism, neurochemistry, and cognitive behavior in a small mammal.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Bei Bo,Xue-Ying Zhang,Kevin D Kohl,Jing Wen,Shuang-Jie Tian,De-Hua Wang

Journal of neurochemistry 154:218-234 PubMed32096869

2020

Dopaminergic afferents from midbrain to dorsolateral bed nucleus of stria terminalis inhibit release and expression of corticotropin-releasing hormone in paraventricular nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Di,Ya Wang,Yajie Zhang,Sha Sha,Yanying Zeng,Ling Chen

Journal of molecular endocrinology 59:365-375 PubMed28912247

2017

miR-212 mediates counter-regulation on CRH expression and HPA axis activity in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiao Tang,Xingjian Cai,Hongwei Zhang,Hui Shen,Wanyin Wang,Zhilei Shen,Wei Gu,Changquan Ling,Min Li
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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