Anti-CREBBP 抗体 [CBP5I001] (ab50702)
Key features and details
- Mouse monoclonal [CBP5I001] to CREBBP
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CREBBP antibody [CBP5I001]
CREBBP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CBP5I001] to CREBBP -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human CREBBP.
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ポジティブ・コントロール
- HeLa whole cell lysate (ab150035); NIH3T3 whole cell lysate. IHC-P: Human pancreas FFPE tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted(approximately 95%) fetal bovine serum, and filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
CBP5I001 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50702の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/50 - 1/100. Predicted molecular weight: 265 kDa.
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ICC/IF |
1/5.
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特記事項 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/50 - 1/100. Predicted molecular weight: 265 kDa. |
ICC/IF
1/5. |
ターゲット情報
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機能
Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300. -
関連疾患
Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. -
配列類似性
Contains 1 bromo domain.
Contains 1 KIX domain.
Contains 2 TAZ-type zinc fingers.
Contains 1 ZZ-type zinc finger. -
ドメイン
The KIX domain mediates binding to HIV-1 Tat. -
翻訳後修飾
Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX. -
細胞内局在
Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1387 Human
- Entrez Gene: 12914 Mouse
- Omim: 600140 Human
- SwissProt: Q92793 Human
- SwissProt: P45481 Mouse
- Unigene: 459759 Human
- Unigene: 132238 Mouse
- Unigene: 392384 Mouse
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別名
- CBP antibody
- CBP_HUMAN antibody
- CREB binding protein antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
Lane 2: CREBBP knockout HAP1 whole cell lysate (40 µg)
Lane 3: HeLa whole cell lysate (40 µg)
Lane 4: NIH3T3 whole cell lysate (40 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab50702 observed at 265 kDa. Red - loading control, ab176560, observed at 50 kDa.
ab50702 was shown to specifically recognize CREBBP in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when CREBBP knockout samples were examined. Wild-type and CREBBP knockout samples were subjected to SDS-PAGE. Ab50702 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/100 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody [CBP5I001] (ab50702)
IHC image of CREBBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50702, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Staining of HeLa cell lysate with ab50702 at a dilution of 1/5 and Goat anti-mouse secondary at 1/200.
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Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/30 dilution + RIPA lysate of HeLa cells at 500 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa -
Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa -
Anti-CREBBP antibody [CBP5I001] (ab50702) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 265 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (13)
ab50702 は 13 報の論文で使用されています。
- Feng D et al. CBP-mediated Wnt3a/ß-catenin signaling promotes cervical oncogenesis initiated by Piwil2. Neoplasia 23:1-11 (2021). PubMed: 33190089
- Feng D et al. Wnt3a/β-Catenin/CBP Activation in the Progression of Cervical Intraepithelial Neoplasia. Pathol Oncol Res 27:609620 (2021). PubMed: 34257574
- Li K & Wang Z Speckles and paraspeckles coordinate to regulate HSV-1 genes transcription. Commun Biol 4:1207 (2021). PubMed: 34675360
- Gabata R et al. Anti-tumor Activity of the Small Molecule Inhibitor PRI-724 Against ß-Catenin-activated Hepatocellular Carcinoma. Anticancer Res 40:5211-5219 (2020). PubMed: 32878809
- Wang Z et al. Modulation of SRSF2 expression reverses the exhaustion of TILs via the epigenetic regulation of immune checkpoint molecules. Cell Mol Life Sci 77:3441-3452 (2020). PubMed: 31838573