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Anti-CRALBP 抗体 [B2] - BSA and Azide free (ab264522)

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Western blot - Anti-CRALBP antibody [B2] - BSA and Azide free (ab264522)

    Key features and details

    • Mouse monoclonal [B2] to CRALBP - BSA and Azide free
    • Suitable for: WB, IHC-P
    • Reacts with: Mouse, Rat
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    HRP

    製品の概要

    • 製品名

      Anti-CRALBP antibody [B2] - BSA and Azide free
      CRALBP 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [B2] to CRALBP - BSA and Azide free

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WB, IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat
      交差が予測される動物種: Cow, Human, Pig, Monkey

    • 免疫原

      Recombinant full length protein corresponding to Human CRALBP.

    • ポジティブ･コントロール

      • This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina.

    • 特記事項

      ab264522 is the carrier-free version of ab15051.

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

      This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab264522の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
    IHC-P
    Use at an assay dependent concentration.
    特記事項
    WB
    Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.

    • 組織特異性

      Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.

    • 関連疾患

      Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
      Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
      Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
      Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

    • 配列類似性

      Contains 1 CRAL-TRIO domain.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Cellular retinaldehyde binding protein 1 antibody
      • Cellular retinaldehyde binding protein antibody
      • Cellular retinaldehyde-binding protein antibody
      • MGC3663 antibody
      • Retinaldehyde binding protein 1 antibody
      • Retinaldehyde-binding protein 1 antibody
      • RLBP 1 antibody
      • RLBP1 antibody
      • RLBP1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-CRALBP antibody [B2] - BSA and Azide free (ab264522)
      Western blot - Anti-CRALBP antibody [B2] - BSA and Azide free (ab264522)
      All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml

      Lane 1 : Rat eye tissue lysate - total protein (ab4035)
      Lane 2 : Rat retina tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 36 kDa


      Exposure time: 30 seconds


      This data was developed using the same antibody clone in a different buffer formulation containing PBS, L-Arginine and sodium azide (ab15051)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab264522 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab264522 は論文での使用が確認できていません。

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