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Anti-CRALBP 抗体 [B2] (ab15051)

Reviews (11)Q&A (6)References (106)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (ab15051)
  • Western blot - Anti-CRALBP antibody [B2] (ab15051)

Key features and details

  • Mouse monoclonal [B2] to CRALBP
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

HRP

製品の概要

  • 製品名

    Anti-CRALBP antibody [B2]
    CRALBP 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [B2] to CRALBP

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Human, Pig, Monkey

  • 免疫原

    Recombinant full length protein corresponding to Human CRALBP.
    Database link: P12271

  • ポジティブ･コントロール

    • This antibody gave a positive signal in the following tissue lysates: rat eye; rat retina. FFPE Mouse eye tissue sections.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    B2

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab15051の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (4)
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).
特記事項
IHC-P
Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 機能

    Soluble retinoid carrier essential the proper function of both rod and cone photoreceptors. Participates in the regeneration of active 11-cis-retinol and 11-cis-retinaldehyde, from the inactive 11-trans products of the rhodopsin photocycle and in the de novo synthesis of these retinoids from 11-trans metabolic precursors. The cycling of retinoids between photoreceptor and adjacent pigment epithelium cells is known as the 'visual cycle'.

  • 組織特異性

    Retina and pineal gland. Not present in photoreceptor cells but is expressed abundantly in the adjacent retinal pigment epithelium (RPE) and in the Mueller glial cells of the retina.

  • 関連疾患

    Defects in RLBP1 are a cause of retinitis pigmentosa autosomal recessive (ARRP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in RLBP1 are the cause of Bothnia retinal dystrophy (BRD) [MIM:607475]; also known as Vasterbotten dystrophy. Affected individuals show night blindness from early childhood with features consistent with retinitis punctata albescens and macular degeneration.
    Defects in RLBP1 are the cause of rod-cone dystrophy Newfoundland (NFRCD) [MIM:607476]. NFRCD is a retinal dystrophy reminiscent of retinitis punctata albescens but with a substantially lower age at onset and more-rapid and distinctive progression. Rod-cone dystrophies results from initial loss of rod photoreceptors, later followed by cone photoreceptors loss.
    Defects in RLBP1 are a cause of fundus albipunctatus (FA) [MIM:136880]. FA is a rare form of stationary night blindness characterized by a delay in the regeneration of cone and rod photopigments.

  • 配列類似性

    Contains 1 CRAL-TRIO domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Cellular retinaldehyde binding protein 1 antibody
    • Cellular retinaldehyde binding protein antibody
    • Cellular retinaldehyde-binding protein antibody
    • MGC3663 antibody
    • Retinaldehyde binding protein 1 antibody
    • Retinaldehyde-binding protein 1 antibody
    • RLBP 1 antibody
    • RLBP1 antibody
    • RLBP1_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (ab15051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRALBP antibody [B2] (ab15051)

    Immunohistochemistry image of CRALBP staining in a section of formalin-fixed paraffin-embedded normal mouse eye performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab15051, 0.5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-CRALBP antibody [B2] (ab15051)
    Western blot - Anti-CRALBP antibody [B2] (ab15051)
    All lanes : Anti-CRALBP antibody [B2] (ab15051) at 1 µg/ml

    Lane 1 : Rat eye tissue lysate - total protein (ab4035)
    Lane 2 : Rat retina tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 36 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

参考文献 (106)

ab15051 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab15051 は 106 報の論文で使用されています。

  • Albertos-Arranz H  et al. Microglia activation and neuronal alterations in retinas from COVID-19 patients: correlation with clinical parameters. Eye Vis (Lond) 10:12 (2023). PubMed: 36855168
  • Boon N  et al. Characterization and AAV-mediated CRB gene augmentation in human-derived CRB1KO and CRB1KOCRB2+/- retinal organoids. Mol Ther Methods Clin Dev 31:101128 (2023). PubMed: 37886604
  • Chen HY  et al. Reserpine maintains photoreceptor survival in retinal ciliopathy by resolving proteostasis imbalance and ciliogenesis defects. Elife 12:N/A (2023). PubMed: 36975211
  • Pan D  et al. CRX haploinsufficiency compromises photoreceptor precursor translocation and differentiation in human retinal organoids. Stem Cell Res Ther 14:346 (2023). PubMed: 38049871
  • Martínez-Gil N  et al. Ischemia-Reperfusion Increases TRPM7 Expression in Mouse Retinas. Int J Mol Sci 24:N/A (2023). PubMed: 38003256
View all Publications for this product

レビューと Q&A

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11-17 of 17 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CRALBP antibody [B2]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Retina)
Specification
Retina
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

投稿 Jun 26 2012

Immunohistochemistry (Frozen sections) abreview for Anti-CRALBP antibody [B2]

 Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Neuroretina)
Specification
Neuroretina
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton x100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. I Fernandez-Bueno

Verified customer

投稿 Apr 07 2009

Question

Dear
Thank you very much for your quick reply.
Actually, I look on your website and i did not see other CRALBP antibody suitable for IHC-P Therefore, I would ask for another CRALBP, the same reference that I already used, but another batch.
Could you tell me when I can expect the delivery of it?
Thanks in advance for your answer,

Read More
Answer

As requested, I have issued a free of charge replacement with the order number XXXXX for the antibody ab15051 from a different lot to the last one received.

To check the status of the order please contact our Customer Service team and reference this number. You will soon receive a confirmation informing about the delivery date.

I hope this new vial works as expected. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Dear,
Please find in attachment my comment on the questionnaire and a picture.
Thanks in advance for your help.
Best regards,

Read More
Answer

I appreciate the time taken to submit further information to us.

Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered.

In order to optimise the protocol and obtain better results from this antibody I would suggest the following steps:

- For this particular antibody a specific antigen retrieval method is recommended. We suggest performing heat mediated antigen retrieval using Citrate buffer pH 6 before the staining. The times for retrieving the antigen should be optimise in each case

The standard recommended time is 20 minutes, less than 20 minutes may leave the antigens under retrieved, leading to weak staining. More than 20 minutes may leave them over-retrieved, leading to nonspecific background staining and also increasing the chances of sections dissociating from the slides. A control experiment is recommended beforehand, where slides of the same tissue section are retrieved for 5, 10, 15, 20, 25 and 30 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. In the following link you will find useful information for the different antigen retrieval methods:

https://www.abcam.com/index.html?pageconfig=resource&rid=11488#notes2

- I would also suggest changing the blocking conditions, using BSA (1%-3%) for 15minutes at room temperature.

- Have you checked the secondary and substrate are working correctly and they are not giving similar problems with other primaries?

I hope this information is helpful. Should the suggestions not improve the results, please do not hesitate to contact me again and I will try to provide further help.

Read More

Question

Dear,
We tested this product, on paraffin cross section and obtained negative results, this at your recommended concentration.
Since your antibody is descrived to be suitable for paraffin cross section (Muller cells staining), can we be refunded or receive another vial?
Thanks in advance for your answer,

Read More
Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

In order to better understand the problem, I would like to send you a questionnaire with some questions about the protocol used, as well as the order details. All this information is crucial to help us understand the possible causes of the problem. Any images are highly appreciated.

Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. If no suggestions can be made to the protocol, and the antibody was purchased within the last 6 months, it is covered by our Abpromise guarantee, and therefore I can offer you a free of charge replacement or a credit note for it.

I look forward to receiving your reply. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

Dear,
Could you send me the MSDS of this product :
Anti-CRALBP antibody [B2] (ab15051)
Thanks in advance for your answer,
Best regards,

Read More
Answer

Thank you for contacting us.



Abcam provides MSDS for those products containing hazardous components. It is not a legal requirement to provide MSDS for those products not considered being hazardous, but if you would need the safety datasheet anyway, please do not hesitate to let me know so we can create it for you.



I hope this helps. Please let us know if we can assist you further.

Read More

Question

The antibody to CRALBP was generated by J Saari. The antibody you cell also has reference to the same researcher. Do your and J Saari's antibodies recognize the same antigen? Are they the same product?

Read More
Answer

Thank you for your enquiry. Yes, I can confirm that this antibody was applied in the following publication; Bunt-Milam AH & Saari JC Immunocytochemical localization of two retinoid-binding proteins in vertebrate retina. J Cell Biol 97:703-12 (1983). PubMed: 6350319 Therefore this antibody recognizes the same antigen. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

11-17 of 17 Abreviews or Q&A

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