Name: Anti-CPT1A antibody [EPR21843-71-2F]
Brand: RabMAb
SKU: ab234111
Rating: 4 (1 reviews)

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Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21843-71-2F] to CPT1A
Suitable for: Flow Cyt (Intra), WB, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-CPT1A antibody [EPR21843-71-2F]
CPT1A 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR21843-71-2F] to CPT1A
由来種

Rabbit
特異性

We detected weak cross-reactivity with CPT1B with the recombinant protein only. Our WB images were generated by testing unboiled samples only.
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Wild-type HAP1 whole cell lysate; HEK-293T, HeLa, SK-OV-3, C6 and MCF7 whole cell lysates; Mouse, rat and human kidney lysates. IHC-P: Human kidney, ovarian carcinoma and heart tissues; Mouse and rat kidney tissues. Flow Cyt (intra): SK-OV-3 cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR21843-71-2F
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-CPT1A antibody [EPR21843-71-2F] - BSA and Azide free (ab235841)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human CPT1A knockout HEK-293T cell line (ab266319)
KO cell lysates
- Human CPT1A knockout HEK-293T cell lysate (ab256880)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab234111の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/50.
WB		1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

特記事項
Flow Cyt (Intra) 1/50.
WB 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

組織特異性

Strong expression in kidney and heart, and lower in liver and skeletal muscle.
パスウェイ

Lipid metabolism; fatty acid beta-oxidation.
関連疾患

Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood.
配列類似性

Belongs to the carnitine/choline acetyltransferase family.
細胞内局在

Mitochondrion outer membrane.
Target information above from: UniProt accession P50416 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1374 Human
- Entrez Gene: 12894 Mouse
- Entrez Gene: 25757 Rat
- Omim: 600528 Human
- SwissProt: P50416 Human
- SwissProt: P97742 Mouse
- SwissProt: P32198 Rat
- Unigene: 503043 Human
- Unigene: 18522 Mouse
- Unigene: 2856 Rat
see all
別名
- Carnitine O palmitoyltransferase 1 liver isoform antibody
- Carnitine O palmitoyltransferase I antibody
- Carnitine O palmitoyltransferase I liver isoform antibody
- Carnitine O-palmitoyltransferase 1 antibody
- Carnitine O-palmitoyltransferase I antibody
- Carnitine palmitoyltransferase 1A (liver) antibody
- Carnitine palmitoyltransferase 1A antibody
- Carnitine palmitoyltransferase I antibody
- Carnitine palmitoyltransferase I liver antibody
- CPT 1 antibody
- CPT I antibody
- CPT1 antibody
- CPT1 L antibody
- CPT1-L antibody
- Cpt1a antibody
- CPT1A_HUMAN antibody
- CPTI antibody
- CPTI-L antibody
- L CPT1 antibody
- liver isoform antibody
see all

Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution

Lane 1 : Human kidney tissue lysate
Lane 2 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 92 seconds

Blocking and diluting buffer: 5% NFDM /TBST

CPT1A is strongly expressed in kidney and heart, and lower in liver and skeletal muscle.

We recommend loading higher amount of lysate or using lower antibody dilution to detect signal in liver lysate.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 180 seconds

Blocking and diluting buffer: 5% NFDM/TBST

CPT1A is strongly expressed in kidney and heart, and lower in liver and skeletal muscle.

We recommend loading higher amount of lysate or using lower antibody dilution to detect signal in HepG2 lysate.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : CPT1A knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 88 kDa
Observed band size: 88 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab234111 observed at 88 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab234111 was shown to react with CPT1A in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266319 (knockout cell lysate ab256880) was used. Wild-type HEK-293T and CPT1A knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab234111 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat kidney lysate
Lane 5 : Human kidney lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 7 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-4 & 6-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time : Lanes 1 and 2: 3 minutes; Lane 3: 37 seconds; Lane 4: 114 seconds; Lane 5: 15 seconds; Lanes 6 and 7: 92 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in rat kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse kidney (PMID: 18192268; PMID: 28956034; PMID: 15363638; PMID: 8679700) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining in human ovarian carcinoma (PMID: 26716645) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized SK-OV-3 (human ovarian cancer cell line) cell line labeling CPT1A with ab234111 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

All lanes : Anti-CPT1A antibody [EPR21843-71-2F] (ab234111) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CPT1A knockout HAP1 whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : SK-OV-3 (human ovarian cancer cell line) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 88 kDa
Observed band size: 88 kDa

Exposure time: 92 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

ab234111 was shown to specifically react with CPT1A in wild-type HAP1 cells as signal was lost in CPT1A knockout cells. Wild-type and CPT1A knockout samples were subjected to SDS-PAGE. ab234111 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

Immunohistochemical analysis of paraffin-embedded human heart tissue labeling CPT1A with ab234111 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human heart is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-CPT1A antibody [EPR21843-71-2F] (ab234111)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab234111 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab234111 は 34 報の論文で使用されています。

Ding S et al. Deficiency of angiopoietin-like 4 enhances CD8+ T cell bioactivity via metabolic reprogramming for impairing tumour progression. Immunology 170:28-46 (2023). PubMed: 37094816
Okada N et al. NFYA promotes malignant behavior of triple-negative breast cancer in mice through the regulation of lipid metabolism. Commun Biol 6:596 (2023). PubMed: 37268670
Zhu Q et al. Virgin Camellia Seed Oil Improves Glycolipid Metabolism in the Kidney of High Fat-Fed Rats through AMPK-SREBP Pathway. Nutrients 15:N/A (2023). PubMed: 38068746
Zhu KG et al. The microprotein encoded by exosomal lncAKR1C2 promotes gastric cancer lymph node metastasis by regulating fatty acid metabolism. Cell Death Dis 14:708 (2023). PubMed: 37903800
Meng X et al. Lipid metabolism marker CD36 is associated with 18FDG-PET/CT false negative lymph nodes in head and neck squamous cell carcinoma. Front Oncol 13:1156527 (2023). PubMed: 37207149

View all Publications for this product

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

Application

Flow Cytometry

Sample

Mouse Cell (Splenocytes)

Permeabilization

Yes - ebio fix/perm

Gating Strategy

lymphocytes, single cells

Specification

Splenocytes

Preparation

Cell harvesting/tissue preparation method: Collagenase digestion
Sample buffer: PBS + 2% FCS

Fixation

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Graham Heieis

Verified customer

投稿 May 17 2021

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Related Products

アプリケーション

The Abpromise guarantee

ターゲット情報

組織特異性

パスウェイ

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (34)

レビューと Q&A

Flow Cytometry abreview for Anti-CPT1A antibody [EPR21843-71-2F]