Anti-CPSF1 抗体 (ab81552)
Key features and details
- Rabbit polyclonal to CPSF1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CPSF1 antibody -
製品の詳細
Rabbit polyclonal to CPSF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide corresponding to Human CPSF1 aa 1350-1450.
Database link: Q10570 -
ポジティブ･コントロール
- HeLa, 293T and NIH3T3 whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 161 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
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IHC-P
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 161 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
CPSF1 (Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity factor (CPSF) complex. It plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction. -
細胞内局在
Nucleus, nucleoplasm. -
参照データベース
- Entrez Gene: 29894 Human
- Entrez Gene: 94230 Mouse
- Entrez Gene: 366952 Rat
- Omim: 606027 Human
- SwissProt: Q10570 Human
- SwissProt: Q9EPU4 Mouse
- SwissProt: B5DEL2 Rat
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別名
- Cleavage and polyadenylation specificity factor 160 kDa subunit antibody
- Cleavage and polyadenylation specificity factor subunit 1 antibody
- CPSF 160 kDa subunit antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (ab81552)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling CPSF1 with ab81552 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CPSF1 antibody (ab81552)All lanes : Anti-CPSF1 antibody (ab81552) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 161 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds -
Immunoprecipitation - Anti-CPSF1 antibody (ab81552)HeLa cell lysate (1mg) was immunoprecipitated using ab81552 at 3µg/mg lysate (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab81552 at 1µg/ml. Bands were detected by chemiluminescence with an exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF1 antibody (ab81552)IHC image of ab81552 staining in Human Breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81552, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab81552 は 5 報の論文で使用されています。
- Xia L et al. m6A-induced repression of SIAH1 facilitates alternative splicing of androgen receptor variant 7 by regulating CPSF1. Mol Ther Nucleic Acids 28:219-230 (2022). PubMed: 35402071
- Kang W et al. CPSF1 positively regulates NSDHL by alternative polyadenylation and promotes gastric cancer progression. Am J Cancer Res 12:4566-4583 (2022). PubMed: 36381317
- Takahashi H et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun 11:1063 (2020). PubMed: 32102997
- Sakai A et al. Aberrant expression of CPSF1 promotes head and neck squamous cell carcinoma via regulating alternative splicing. PLoS One 15:e0233380 (2020). PubMed: 32437477
- Yu S et al. SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3' end processing. Nucleic Acids Res 46:8557-8573 (2018). PubMed: 29860334