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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CPS1 抗体 [EPR7493-29] (ab155083)

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Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
  • Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-29] (ab155083)
  • Anti-CPS1 antibody [EPR7493-29] (ab155083)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7493-29] to CPS1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CPS1 antibody [EPR7493-29]
    CPS1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7493-29] to CPS1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF

  • 種交差性

    交差種: Rat, Human
    交差が予測される動物種: Mouse

  • 免疫原

    Synthetic peptide within Human CPS1. The exact sequence is proprietary.
    Database link: P31327

  • ポジティブ･コントロール

    • WB: HeLa cell lysate and human fetal liver and rat liver tissue lysates. IHC-P: Human liver tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab155083の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 165 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 165 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt or ICC/IF.

ターゲット情報

  • 機能

    Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

  • 組織特異性

    Primarily in the liver and small intestine.

  • 関連疾患

    Defects in CPS1 are the cause of carbamoyl phosphate synthetase 1 deficiency (CPS1D) [MIM:237300]. CPS1D is an autosomal recessive disorder of the urea cycle causing hyperammonemia. Clinical features include protein intolerance, intermittent ataxia, seizures, lethargy, developmental delay and mental retardation.
    Note=Genetic variations in CPS1 influence the availability of precursors for nitric oxide (NO) synthesis and play a role in clinical situations where endogenous NO production is critically important, such as neonatal pulmonary hypertension, increased pulmonary artery pressure following surgical repair of congenital heart defects or hepatovenocclusive disease following bone marrow transplantation. Infants with neonatal pulmonary hypertension homozygous for Thr-1406 have lower L-arginine concentrations than neonates homozygous for Asn-1406.

  • 配列類似性

    Contains 2 ATP-grasp domains.
    Contains 1 glutamine amidotransferase type-1 domain.

  • ドメイン

    The type-1 glutamine amidotransferase domain is defective.

  • 細胞内局在

    Mitochondrion.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Carbamoyl phosphate synthase [ammonia] antibody
    • Carbamoyl phosphate synthase [ammonia] mitochondrial antibody
    • Carbamoyl phosphate synthase antibody
    • Carbamoyl phosphate synthetase 1 antibody
    • Carbamoyl phosphate synthetase 1 mitochondrial antibody
    • Carbamoyl phosphate synthetase I antibody
    • Carbamoyl-phosphate synthase [ammonia] antibody
    • Carbamoyl-phosphate synthetase I antibody
    • Carbamoylphosphate synthase antibody
    • Carbamoylphosphate synthetase 1 antibody
    • Carbamoylphosphate synthetase I antibody
    • CPS 1 antibody
    • Cps1 antibody
    • CPSase 1 antibody
    • CPSase I antibody
    • CPSASE1 antibody
    • CPSM_HUMAN antibody
    • mitochondrial antibody
    • MS738 antibody
    see all

画像

  • Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
    Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
    All lanes : Anti-CPS1 antibody [EPR7493-29] (ab155083) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CPS1 knockout HeLa cell lysate
    Lane 3 : Human liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 165 kDa
    Observed band size: 165 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab155083 observed at 165 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab155083 Anti-CPS1 antibody [EPR7493-29] was shown to specifically react with CPS1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261809 (knockout cell lysate ab257121) was used. Wild-type and CPS1 knockout samples were subjected to SDS-PAGE. ab155083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
    Western blot - Anti-CPS1 antibody [EPR7493-29] (ab155083)
    All lanes : Anti-CPS1 antibody [EPR7493-29] (ab155083) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Human fetal liver lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 165 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-29] (ab155083)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPS1 antibody [EPR7493-29] (ab155083)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue, labeling CPS1 with ab155083 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-CPS1 antibody [EPR7493-29] (ab155083)
    Anti-CPS1 antibody [EPR7493-29] (ab155083)

参考文献 (1)

ab155083 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab155083 は 1 報の論文で使用されています。

  • Chen Z  et al. The activity of the carbamoyl phosphate synthase 1 promoter in human liver-derived cells is dependent on hepatocyte nuclear factor 3-beta. J Cell Mol Med 21:2036-2045 (2017). PubMed: 28272778

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