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Anti-CPI-17 (phospho T38) 抗体 (ab52174)

Reviews (2)Q&A (2)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (ab52174)
  • Western blot - Anti-CPI-17 (phospho T38) antibody (ab52174)

Key features and details

  • Rabbit polyclonal to CPI-17 (phospho T38)
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CPI-17 (phospho T38) antibody
    CPI-17 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to CPI-17 (phospho T38)

  • 由来種

    Rabbit

  • 特異性

    ab52174 detects endogenous levels of CPI17a only when phosphorylated at threonine 38.

  • アプリケーション

    適用あり: IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Synthetic peptide corresponding to Human CPI-17 aa 1-100 (phospho T38).
    Database link: Q96A00

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab52174 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab52174の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
WB (2)
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
特記事項
IHC-P
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

ターゲット情報

  • 機能

    Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.

  • 組織特異性

    Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

  • 配列類似性

    Belongs to the PP1 inhibitor family.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 17 kDa PKC-potentiated inhibitory protein of PP1 antibody
      • 17-KDa protein antibody
      • 17kDa PKC potentiated inhibitory protein of PP1 antibody
      • CPI 17 alpha antibody
      • CPI 17 antibody
      • CPI 17alpha antibody
      • CPI-17 antibody
      • CPI17 alpha antibody
      • CPI17 antibody
      • PKC potentiated inhibitory protein of PP1 antibody
      • PKC potentiated inhibitory protein of PP1, 17-KD antibody
      • PP14A_HUMAN antibody
      • PPP1INL antibody
      • Ppp1r14a antibody
      • Protein kinase C-potentiated inhibitor of protein phosphatase 1, 17-KD antibody
      • Protein kinase C-potentiated inhibitor protein of 17 kDa antibody
      • Protein phosphatase 1 antibody
      • Protein phosphatase 1 regulatory (inhibitor) subunit 14A antibody
      • Protein phosphatase 1 regulatory subunit 14A antibody
      • Regulatory subunit 14A antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (ab52174)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPI-17 (phospho T38) antibody (ab52174)

      ab52174 at 1/50 dilution staining CPI-17 in human breast carcinoma by Immunohistochemistry, paraffin embedded tissue, in the presence (right image) and absence (left image) of the immunising peptide.

    • Western blot - Anti-CPI-17 (phospho T38) antibody (ab52174)
      Western blot - Anti-CPI-17 (phospho T38) antibody (ab52174)

      Western blot analysis of extracts from RAW264.7 cells, using CPI-17  (Phospho-Thr38) (ab52174)Antibody. The lane on the right is treated with the synthesized peptide.

    参考文献 (5)

    ab52174 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab52174 は 5 報の論文で使用されています。

    • Lin Z  et al. Improving Small Intestinal Motility in Experimental Acute Necrotising Pancreatitis by Modulating the CPI-17/MLCP Pathway Using Chaiqin Chengqi Decoction. Evid Based Complement Alternat Med 2020:9189457 (2020). PubMed: 32104199
    • Ge J  et al. S-adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient receptor protein 3/protein kinase Cß/C-kinase-activated protein phosphatase-1 inhibitor of 17 kDa signaling pathway. Exp Ther Med 16:103-112 (2018). PubMed: 29896230
    • Chen J  et al. Roles and mechanisms of TRPC3 and the PLC?/PKC/CPI-17 signaling pathway in regulating parturition. Mol Med Rep 17:898-910 (2018). PubMed: 29115500
    • Yin Y  et al. Expression of MYPT1, CPI-17 and MLC20 in ileum of neonatal mouse NEC model and its significance. Exp Ther Med 14:2221-2227 (2017). PubMed: 28962146
    • Kang S  et al. Dysfunction of vascular smooth muscle and vascular remodeling by simvastatin. Toxicol Sci 138:446-556 (2014). Rat . PubMed: 24449418

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-CPI-17 (phospho T38) antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Aortic smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing (gel 15%)
    Loading amount
    17.1 µg
    Treatment
    10μM 5-HT for 5 mins
    Specification
    Aortic smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 11 2022

    Western blot abreview for Anti-CPI17 alpha (phospho T38) antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Myometrial smooth muscle cells)
    Loading amount
    15 µg
    Specification
    Myometrial smooth muscle cells
    Treatment
    1 or 10 nM oxytocin for 1 minute, and 1µM phorbol 12-myristate 13-acetate for 10 minutes
    Gel Running Conditions
    Reduced Denaturing (13% tris-glycine SDS-PAGE gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 10 2009

    Question

    Dear Sir/Madam, We would like to know if this Anti-CPI17 alpha (phospho T38) antibody has been detected in C2C12 cellular extracts. If so, could you please send me some information regarding the detecting with this antibody. Looking forward to hearing from you.

    Read More
    Answer

    Thank you for contacting us.

    We have not specifically tested ab52147 on C2C12 cells. However, we do have customer feedback regarding the use of this antibody to detect the phosphorylated protein in treated myometrial smooth muscle cells.

    https://www.abcam.com/CPI-17-phospho-T38-antibody-ab52174.html&tab=abreviews&intabreviewid=13947

    In his treatment, this customer used oxytocin, which we carry as ab120186 and phorbol 12-myristate 13-acetate (PMA), which we carry as ab120297.

    https://www.abcam.com/Oxytocin-ab120186.html

    https://www.abcam.com/Phorbol-12-myristate-13-acetate-PMA-ab120297.html

    I do not know enough about the protein to tell you definitively whether or not this protein is endogenously phosphorylated in C2C12 cells. I do have an image of ab52174 used in WB with untreated RAW264.7 cells though, which we sell as ab7187. I'll be sure to get that WB image added to the datasheet online, but it will take a few days to appear.

    https://www.abcam.com/RAW-264-7-Mouse-leukaemic-monocyte-macrophage-cell-line-Whole-Cell-Lysate-ab7187.html

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Sir/Madam,

    We would like to know if this Anti-CPI17 alpha (phospho T38) antibody has been detected in C2C12 cellular extracts. If so, could you please send me some information regarding the detecting with this antibody. Looking forward to hearing from you.

    Read More
    Answer

    Thank you for contacting us.
    This antibody has been validated for Western blot using RAW264.7 cell lysate. It has not been tested against other mouse cell lines, such as C2C12.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

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