Anti-CPEB3 抗体 (ab10883)
Key features and details
- Rabbit polyclonal to CPEB3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CPEB3 antibody -
製品の詳細
Rabbit polyclonal to CPEB3 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- ICC/IF: SKNSH cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10883の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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配列類似性
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. - Information by UniProt
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参照データベース
- Entrez Gene: 22849 Human
- Entrez Gene: 208922 Mouse
- SwissProt: Q8NE35 Human
- SwissProt: Q7TN99 Mouse
- Unigene: 131683 Human
- Unigene: 701732 Human
- Unigene: 391176 Mouse
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別名
- CPE-binding protein 3 antibody
- CPE-BP3 antibody
- CPEB 3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CPEB3 antibody (ab10883)
ab10883 staining CPEB3 in SKNSH cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab10883 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-CPEB3 antibody (ab10883)Lane 1 : Anti-CPEB3 antibody (ab10883) at 1/500 dilution
Lane 2 : Anti-CPEB3 antibody (ab10883) at 1/1000 dilution
All lanes : Mouse Brain lysate
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution
Predicted band size: 75 kDa
Exposure time: 3 minutesLanes 1 to 2
Lane 1: 5µ
Lane 2: 5µ
Exposure time: 3 minutes
Expected molecular weight: 78kDa
Secondary ab: Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed
ab7090 (1/5000)We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
We think that the top band here is the correct band, as it is the same size as the most intense band on the previous blot. The banding pattern is the same on both blots and the lower bands may be clevage products as there is no evidence of CPEB3 isoforms in the literature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab10883 は 17 報の論文で使用されています。
- Winkler I et al. MicroRNA-92a-CPEB3 axis protects neurons against inflammatory neurodegeneration. Sci Adv 9:eadi6855 (2023). PubMed: 38000031
- Hwang JY et al. CPEB3-dependent increase in GluA2 subunits impairs excitatory transmission onto inhibitory interneurons in a mouse model of fragile X. Cell Rep 39:110853 (2022). PubMed: 35675768
- Shu Z et al. MiR-9-5p promotes cell proliferation and migration of hepatocellular carcinoma by targeting CPEB3. Biomark Med 15:97-108 (2021). PubMed: 33496636
- He Y et al. Long non-coding RNA MIR22HG inhibits glioma progression by downregulating microRNA-9/CPEB3. Oncol Lett 21:157 (2021). PubMed: 33552275
- Li J et al. Regulation of miR-128 in the nucleus accumbens affects methamphetamine-induced behavioral sensitization by modulating proteins involved in neuroplasticity. Addict Biol 26:e12881 (2021). PubMed: 32058631