Anti-CPEB1 抗体 (ab3465)
Key features and details
- Rabbit polyclonal to CPEB1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CPEB1 antibody
CPEB1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CPEB1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Xenopus laevis -
免疫原
Synthetic peptide corresponding to Human CPEB1 aa 545-562.
Sequence:HSMEGLRHHSPLMRNQKN
(Peptide available asab4988) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/500.
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ICC/IF | (1) |
1/50 - 1/500.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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特記事項 |
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IHC-P
1/50 - 1/500. |
ICC/IF
1/50 - 1/500. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
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機能
Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. -
組織特異性
Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines. -
配列類似性
Belongs to the RRM CPEB family.
Contains 2 RRM (RNA recognition motif) domains. -
ドメイン
The 2 RRM domains and the C-terminal region mediate interaction with CPE-containing RNA. -
翻訳後修飾
Phosphorylated on serine/threonine residues by AURKA/STK6 within positions 166 and 197. Phosphorylation and dephosphorylation on Thr-172 regulates cytoplasmic polyadenylation and translation of CPE-containing mRNAs. Phosphorylation on Thr-172 by AURKA/STK6 and CAMK2A activates CPEB1. Phosphorylation on Thr-172 may be promoted by APLP1. Phosphorylation increases binding to RNA. -
細胞内局在
Cytoplasm > P-body. Cytoplasmic granule. Cell junction > synapse. Membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment. In dendrites (By similarity). Localizes in synaptosomes at dendritic synapses of neurons (By similarity). Strongly enriched in postsynaptic density (PSD) fractions (By similarity). Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity). Membrane-associated (By similarity). Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA/STK6, SYP, etc.) including CPE-containing RNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 64506 Human
- Entrez Gene: 12877 Mouse
- Entrez Gene: 293056 Rat
- Entrez Gene: 399289 Xenopus laevis
- Entrez Gene: 734470 Xenopus laevis
- Omim: 607342 Human
- SwissProt: Q9BZB8 Human
- SwissProt: P70166 Mouse
see all -
別名
- CEBP antibody
- CPE binding protein 1 antibody
- CPE BP1 antibody
see all
画像
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All lanes : Anti-CPEB1 antibody (ab3465) at 1/500 dilution
Lane 1 : HeLa lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit
Developed using the ECL technique.
Predicted band size: 65 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute -
Western blot detection of 1.0 ng of recombinant mouse CPEB1 using ab3465.
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Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human hearat tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry analysis of CPEB showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3465 diluted in 3% BSA-PBS at a dilution of 1/100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
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Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
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Immunofluorescent analysis of CPEB (green) showing staining in the cytoplasm of HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3465 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight®-conjugated secondary antibody in PBS at room temperature in the dark. Actin was stained using Alexa Fluor® 554 (red) and nuclei were stained with Hoechst or DAPI (blue). Images were taken at a magnification of 60x.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab3465 は 6 報の論文で使用されています。
- Hwang JY et al. CPEB3-dependent increase in GluA2 subunits impairs excitatory transmission onto inhibitory interneurons in a mouse model of fragile X. Cell Rep 39:110853 (2022). PubMed: 35675768
- Chen R et al. miR-129-3p alleviates chondrocyte apoptosis in knee joint fracture-induced osteoarthritis through CPEB1. J Orthop Surg Res 15:552 (2020). PubMed: 33228708
- Xu K et al. Depletion of CPEB1 protects against oxidized LDL-induced endothelial apoptosis and inflammation though SIRT1/LOX-1 signalling pathway. Life Sci 239:116874 (2019). PubMed: 31521690
- Margvelani G et al. Micro-RNAs, their target proteins, predispositions and the memory of filial imprinting. Sci Rep 8:17444 (2018). PubMed: 30487553
- Chen J et al. Genome-wide analysis of translation reveals a critical role for deleted in azoospermia-like (Dazl) at the oocyte-to-zygote transition. Genes Dev 25:755-66 (2011). WB ; Mouse . PubMed: 21460039
- Blanchard Z et al. Geminin overexpression induces mammary tumors via suppressing cytokinesis. Oncotarget 2:1011-27 (2011). PubMed: 22184288