Anti-COX6B1 抗体 [3F9D3D11AF6] (ab110266)
![Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.co.jp/ps/products/110/ab110266/Images/ab110266-196280-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)")
Key features and details
- Mouse monoclonal [3F9D3D11AF6] to COX6B1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-COX6B1 antibody [3F9D3D11AF6]
COX6B1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3F9D3D11AF6] to COX6B1 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Cow, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human, cow, rat and mouse heart. IF/ICC: HeLa cell line.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
特記事項（精製）
Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
3F9D3D11AF6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110266の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa. |
ターゲット情報
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機能
Connects the two COX monomers into the physiological dimeric form. -
関連疾患
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome. -
配列類似性
Belongs to the cytochrome c oxidase subunit 6B family. -
細胞内局在
Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 100270792 Cow
- Entrez Gene: 1340 Human
- Entrez Gene: 110323 Mouse
- Entrez Gene: 502592 Rat
- Omim: 124089 Human
- SwissProt: P00429 Cow
- SwissProt: P14854 Human
- SwissProt: P56391 Mouse
see all -
別名
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)
ICC/IF image of ab110266 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110266, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)](https://www.abcam.co.jp/ps/products/110/ab110266/Images/ab110266-123458-anti-cytochrome-c-oxidase-subunit-vib-antibody-3f9d3d11af6-western-blot.jpg)
Western blot - Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266)All lanes : Anti-COX6B1 antibody [3F9D3D11AF6] (ab110266) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human Heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size: 10 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab110266 は 12 報の論文で使用されています。
- Protasoni M et al. Respiratory supercomplexes act as a platform for complex III-mediated maturation of human mitochondrial complexes I and IV. EMBO J 39:e102817 (2020). PubMed: 31912925
- Cheong A et al. Nuclear-encoded mitochondrial ribosomal proteins are required to initiate gastrulation. Development 147:N/A (2020). PubMed: 32376682
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
- Mohanraj K et al. Inhibition of proteasome rescues a pathogenic variant of respiratory chain assembly factor COA7. EMBO Mol Med 11:N/A (2019). PubMed: 30885959
- Kim SE et al. Upregulation of cytochrome c oxidase subunit 6b1 (Cox6b1) and formation of mitochondrial supercomplexes: implication of Cox6b1 in the effect of calorie restriction. Age (Dordr) 37:9787 (2015). PubMed: 25929654