Anti-COX4I2 抗体 (ab70112)
Key features and details
- Rabbit polyclonal to COX4I2
- Suitable for: WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-COX4I2 antibody -
製品の詳細
Rabbit polyclonal to COX4I2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human COX4I2 aa 31-80 (internal sequence).
Database link: Q96KJ9 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab70112 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70112の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
|
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| ICC/IF |
1/500 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
|
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
組織特異性
Highly expressed in lung. -
関連疾患
Defects in COX4I2 are the cause of exocrine pancreatic insufficiency dyserythropoietic anemia and calvarial hyperostosis (EPIDACH) [MIM:612714]. Patients present with pancreatic insufficiency, intestinal malabsorption, failure to thrive, and anemia soon after birth. -
配列類似性
Belongs to the cytochrome c oxidase IV family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 84701 Human
- Omim: 607976 Human
- SwissProt: Q96KJ9 Human
- Unigene: 277101 Human
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別名
- COX IV-2 antibody
- COX4 2 antibody
- COX4 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-COX4I2 antibody (ab70112)
ab70112 at 1/500 dilution staining COX4I2 in COS7 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Western blot - Anti-COX4I2 antibody (ab70112)All lanes : Anti-COX4I2 antibody (ab70112) at 1/500 dilution
Lane 1 : K562 cell extracts treated
with insulin (0.01U/ml, 15mins)
Lane 2 : K562 cell extracts treated
with insulin (0.01U/ml, 15mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab70112 は 6 報の論文で使用されています。
- Lobo-Jarne T et al. Human COX7A2L Regulates Complex III Biogenesis and Promotes Supercomplex Organization Remodeling without Affecting Mitochondrial Bioenergetics. Cell Rep 25:1786-1799.e4 (2018). PubMed: 30428348
- Almasi S et al. TRPM2 channel-mediated regulation of autophagy maintains mitochondrial function and promotes gastric cancer cell survival via the JNK-signaling pathway. J Biol Chem 293:3637-3650 (2018). PubMed: 29343514
- Zhang X et al. Oxidative stress regulates cellular bioenergetics in esophageal squamous cell carcinoma cell. Biosci Rep 37:N/A (2017). PubMed: 29026004
- Lou PH et al. Loss of Intralipid®- but not sevoflurane-mediated cardioprotection in early type-2 diabetic hearts of fructose-fed rats: importance of ROS signaling. PLoS One 9:e104971 (2014). WB ; Rat . PubMed: 25127027
- Head B et al. Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J Cell Biol 187:959-66 (2009). PubMed: 20038677
- Ahn BH et al. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proc Natl Acad Sci U S A 105:14447-52 (2008). WB ; Mouse . PubMed: 18794531