AB110272

Anti-COX4, mitochondrial 抗体 [1A12A12]

Name: Anti-COX4, mitochondrial 抗体 [1A12A12] (ab110272) | Abcam
Brand: Abcam Limited
SKU: AB110272
Price: 65000 JPY
Availability: InStock
Rating: 3 (1 reviews)

Anti-COX4, mitochondrial antibody [1A12A12]

(1 Review)

(28 Publications)

Mouse Monoclonal COX4, mitochondrial antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 28 publications.

別名を表示する

YGL187C, G1362, COX4, Cytochrome c oxidase polypeptide IV

1 Images

Unknown

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (AB110272)

All lanes:

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (ab110272) at 1 µg/mL

All lanes:

Mitochondria from yeast membrane extract at 20 µg

Predicted band size: 17 kDa

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Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

1A12A12

アイソタイプ

IgG1

軽鎖のタイプ

kappa

キャリアフリー

交差種

Saccharomyces cerevisiae

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態

Liquid

精製度

IgG fraction

精製に関する特記事項

Near homogeneity as judged by SDS-PAGE. ab110272 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

バッファー組成

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

COX4 also known as cytochrome c oxidase subunit IV is a component of the mitochondrial respiratory chain. It has an approximate molecular weight of 17 kDa. COX4 is expressed in high levels in tissues with abundant energy requirements such as heart muscle and brain. This protein is part of the cytochrome c oxidase (complex IV) the last enzyme in the mitochondrial electron transport chain which plays an important role in cellular respiration.

Biological function summary

The cytochrome c oxidase complex facilitates the transfer of electrons from cytochrome c to oxygen ultimately generating water. This process contributes to the proton gradient across the mitochondrial membrane necessary for ATP synthesis. COX4 serves as a regulatory subunit that can influence the efficiency of the electron transfer process. This complex is vital for maintaining the balance of energy production in cells and has a role in adaptive responses to varying energy demands.

Pathways

COX4 functions in the oxidative phosphorylation pathway which is critical for ATP production in eukaryotic cells. This pathway includes several protein complexes with COX4 being a part of the terminal complex IV. It interacts with other proteins such as cytochrome c and ATP synthase linking electron transport to ATP production. In turn this pathway supports various biological functions and maintains cellular energy homeostasis.

Dysfunctional COX4 is associated with mitochondrial diseases that often lead to compromised energy production. Conditions such as mitochondrial myopathy and cytochrome c oxidase deficiency have been linked to functional anomalies in COX4. These disorders can affect muscles and neural tissues leading to symptoms such as muscle weakness and neurological impairments. COX4 acts with proteins like cytochrome c and other subunits of the cytochrome c oxidase complex in these disease mechanisms influencing the severity and progression of these conditions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of COX2 and heme A of COX1 to the active site in COX1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.

See full target information COX4

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5314 PubMed40595511

2025

A constricted mitochondrial morphology formed during respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Manish K Singh,Laetitia Cavellini,Maria Angeles Morcillo-Parra,Christina Kunz,Mickaël Lelek,Perrine Bomme,Aurélia Barascu,Cynthia Alsayyah,Maria Teresa Teixeira,Naïma Belgareh-Touzé,Adeline Mallet,Lea Dietrich,Christophe Zimmer,Mickael M Cohen

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

European journal of histochemistry : EJH 68: PubMed39526436

2024

Activation of Hedgehog pathway by circEEF2/miR-625-5p/TRPM2 axis promotes prostate cancer cell proliferation through mitochondrial stress.

Applications

Unspecified application

Species

Unspecified reactive species

ChenHui Zhu,LiJuan Lin,ChangQing Huang,ZhiHui Wu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2407705 PubMed39447130

2024

Unfractionated Heparin Enhances Sepsis Prognosis Through Inhibiting Drp1-Mediated Mitochondrial Quality Imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixue Liu,He Huang,Dongyao Hou,Shuai Hao,Qiao Guo,Haitang Liao,Rui Song,Yu Tian,Qian Chen,Zhenchun Luo,Daqing Ma,Liangming Liu,Chenyang Duan

Methods in enzymology 700:77-104 PubMed38971613

2024

Using the yeast vacuole as a system to test the lipidic drivers of membrane heterogeneity in living cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Israel Juarez-Contreras,Itay Budin

The Journal of biological chemistry 300:105496 PubMed38013088

2023

Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.

Applications

Unspecified application

Species

Unspecified reactive species

Hyesoo Kim,Itay Budin

The Journal of clinical investigation 132: PubMed35426376

2022

Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Yu Zou,Lin Guo,Bei Zhang,Si Chen,Xin-Rong Wu,Xian-Dong Liu,Xin-Yu Xu,Bin-Yin Li,Shengdi Chen,Nan-Jie Xu,Suya Sun

Clinical science (London, England : 1979) 136:45-60 PubMed34918039

2021

Inhibition of HDAC3 protects against kidney cold storage/transplantation injury and allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xiang,Guie Dong,Jiefu Zhu,Gang Zhang,Zheng Dong

Journal of biochemical and molecular toxicology 36:e22939 PubMed34719845

2021

Genistein suppresses ox-LDL-elicited oxidative stress and senescence in HUVECs through the SIRT1-p66shc-Foxo3a pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Huaping Zhang,Xuefen Pang,Haixia Yu,Hui Zhou

PLoS genetics 17:e1009808 PubMed34665800

2021

Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Corbi,Angelika Amon

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX4, mitochondrial 抗体 [1A12A12]

Anti-COX4, mitochondrial antibody [1A12A12]

Western blot - Anti-COX4, mitochondrial antibody [1A12A12] (AB110272)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

軽鎖のタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (28)

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise