Anti-COX2 / Cyclooxygenase 2 抗体 [SP21] (ab16701)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2
- Suitable for: IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody [SP21]
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP21] to COX2 / Cyclooxygenase 2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details
適用なし: WB -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Rat COX2/ Cyclooxygenase 2 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- IHC-P: Human colon carcinoma tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP21 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16701の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Omim: 600262 Human
- SwissProt: P35354 Human
- SwissProt: Q05769 Mouse
- SwissProt: P35355 Rat
- Unigene: 196384 Human
see all -
別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [SP21] (ab16701)
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma labeling COX2/Cyclooxygenase 2 with ab16701 at 1/100 (6 µg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16701 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab16701 は 7 報の論文で使用されています。
- Madhavan MK et al. Aberrant uterine folding in mice disrupts implantation chamber formation and alignment of embryo-uterine axes. Development 149:N/A (2022). PubMed: 35575097
- Szlendak M et al. Expression of cyclooxygenase-2 and mucin 1 in colorectal cancer. Mol Clin Oncol 13:52 (2020). PubMed: 32874582
- Keren L et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
- Peng YJ et al. Adiponectin and adiponectin receptor 1 overexpression enhance inflammatory bowel disease. J Biomed Sci 25:24 (2018). PubMed: 29540173
- Szylberg L et al. Expression of COX-2, IL-1ß, TNF-a and IL-4 in epithelium of serrated adenoma, adenoma and hyperplastic polyp. Arch Med Sci 12:172-8 (2016). PubMed: 26925134
- Fujita M et al. COX-2 blockade suppresses gliomagenesis by inhibiting myeloid-derived suppressor cells. Cancer Res 71:2664-74 (2011). PubMed: 21324923
- Lu TC et al. Analgesic and Anti-Inflammatory Activities of the Methanol Extract from Pogostemon cablin. Evid Based Complement Alternat Med : (2009). PubMed: 19933324