Anti-COX2 / Cyclooxygenase 2 抗体 (ab52237)
Key features and details
- Rabbit polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COX2 / Cyclooxygenase 2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human COX2/ Cyclooxygenase 2.
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ポジティブ･コントロール
- WB: Human primary myometrial cells whole cell lysate, A549 cells. ICC/IF: A549 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52237の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
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ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5743 Human
- Omim: 600262 Human
- SwissProt: P35354 Human
- Unigene: 196384 Human
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別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/1000 dilution + Human primary myometrial cells whole cell lysate at 20 µg
Secondary
Anti-rabbit IgG, HRP-linked at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab52237) at 1/500 dilution
Lane 1 : A549 (Human lung adenocarcinoma epithelial)
Lane 2 : A549 (Human lung adenocarcinoma epithelial) with Synthesized Peptide
Predicted band size: 69 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)Immunocytochemistry/immunofluorescence analysis of A549 (Human lung adenocarcinoma epithelial) cells labelling COX2 / Cyclooxygenase 2 with ab52237 at dilution of 1/100. No staining was abserved when blocked with synthesized peptide as shown in the inset.
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab52237)This image is courtesy of an anonymous AbreviewAnti-COX2 / Cyclooxygenase 2 antibody (ab52237) (Human mesothelioma whole cell lysate.)
Secondary
Goat anti-rabbit HRP
Predicted band size: 69 kDa
Please see accompanying abreview for additional information
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (96)
ab52237 は 96 報の論文で使用されています。
- Corpuz-Hilsabeck M et al. Dysregulation of Immature Sertoli Cell Functions by Exposure to Acetaminophen and Genistein in Rodent Cell Models. Cells 12:N/A (2023). PubMed: 37443838
- Lee CM et al. Inhibition of lipopolysaccharide-induced inflammation by trophoblast-conditioned medium and trophoblast-derived extracellular vesicles in human middle ear epithelial cells. Sci Rep 13:19822 (2023). PubMed: 37963902
- Takami K et al. Anti-NF-κB peptide derived from nuclear acidic protein attenuates ovariectomy-induced osteoporosis in mice. JCI Insight 8:N/A (2023). PubMed: 37991021
- Oh E et al. WGA-M001, a Mixture of Total Extracts of Tagetes erecta and Ocimum basilicum, Synergistically Alleviates Cartilage Destruction by Inhibiting ERK and NF-κB Signaling. Int J Mol Sci 24:N/A (2023). PubMed: 38139287
- Ghatak S et al. Identification of a Novel Five-Gene Signature as a Prognostic and Diagnostic Biomarker in Colorectal Cancers. Int J Mol Sci 23:N/A (2022). PubMed: 35054980