Anti-COX2 / Cyclooxygenase 2 抗体 (ab23672)
Key features and details
- Goat polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, IHC-P
- Reacts with: Sheep, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-COX2 / Cyclooxygenase 2 antibody
COX2 / Cyclooxygenase 2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to COX2 / Cyclooxygenase 2 -
由来種
Goat -
特異性
ab23672 recognises COX2. It does not cross react with COX1. -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Sheep, Human
交差が予測される動物種: Horse, Cow, Dog, Pig, Mink
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免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
(Peptide available asab49841) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab23672 was purified by affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23672の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa.
|
| IHC-P | (1) |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
翻訳後修飾
S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
- SwissProt: P35354 Human
- SwissProt: P79208 Sheep
see all -
別名
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
画像
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution
Lane 1 : Human COX-1
Lane 2 : Recombinant ovine COX-2 protein
Lane 3 : Recombinant human COX-2 protein
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)Immunohistochemistry analysis of formalin fixed, paraffin-embedded human kidney sections labeling COX2 / Cyclooxygenase 2 with ab23672 at 1/80 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen (DAB). Antigen retrieval was heat mediated using citrate buffer pH 6.0.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab23672 は 24 報の論文で使用されています。
- Kim JE et al. PLPP/CIN-mediated NF2 S10 dephosphorylation distinctly regulates kainate-induced seizure susceptibility and neuronal death through PAK1-NF-κB-COX-2-PTGES2 signaling pathway. J Neuroinflammation 20:99 (2023). PubMed: 37118736
- Zarrough AE et al. Resolvin D1 modulates periodontal ligament fibroblast function. J Periodontol 94:683-693 (2023). PubMed: 36416879
- Zang CX et al. HACE1 negatively regulates neuroinflammation through ubiquitylating and degrading Rac1 in Parkinson's disease models. Acta Pharmacol Sin 43:285-294 (2022). PubMed: 34593974
- Ouyang B et al. miR-146b-5p inhibits tumorigenesis and metastasis of gallbladder cancer by targeting Toll-like receptor 4 via the nuclear factor-?B pathway. Oncol Rep 45:N/A (2021). PubMed: 33649824
- Zhao Z et al. Fecal microbiota transplantation protects rotenone-induced Parkinson's disease mice via suppressing inflammation mediated by the lipopolysaccharide-TLR4 signaling pathway through the microbiota-gut-brain axis. Microbiome 9:226 (2021). PubMed: 34784980