Anti-COX17 抗体 (ab69611)
Key features and details
- Rabbit polyclonal to COX17
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-COX17 antibody -
製品の詳細
Rabbit polyclonal to COX17 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide derived from the N-terminal of human COX17.
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ポジティブ･コントロール
- Human liver carcinoma tissue. Extracts from HeLa, HepG2, 293 and COLO cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69611の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB |
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 7 kDa).
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| IHC-P | (1) |
1/50 - 1/100.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 7 kDa). |
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IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the COX17 family. -
細胞内局在
Mitochondrion intermembrane space. - Information by UniProt
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参照データベース
- Entrez Gene: 10063 Human
- Entrez Gene: 12856 Mouse
- Entrez Gene: 89786 Rat
- Omim: 604813 Human
- SwissProt: Q14061 Human
- SwissProt: P56394 Mouse
- Unigene: 534383 Human
- Unigene: 27396 Mouse
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別名
- COX 17 antibody
- COX17 antibody
- COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) antibody
see all
画像
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Western blot - Anti-COX17 antibody (ab69611)All lanes : Anti-COX17 antibody (ab69611) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from COLO cells
Lane 4 : Extracts from 293 cells
Lane 5 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 7 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX17 antibody (ab69611)ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-COX17 antibody (ab69611)ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab69611 は 5 報の論文で使用されています。
- Zhong Y et al. Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca2+ Homeostasis, and Bcl2 Expression. Mol Nutr Food Res 65:e2001202 (2021). PubMed: 34075698
- Chaphalkar RM et al. Endothelin-1 Mediated Decrease in Mitochondrial Gene Expression and Bioenergetics Contribute to Neurodegeneration of Retinal Ganglion Cells. Sci Rep 10:3571 (2020). PubMed: 32107448
- Wen JJ et al. The Genetic Evidence of Burn-Induced Cardiac Mitochondrial Metabolism Dysfunction. Biomedicines 8:N/A (2020). PubMed: 33287280
- Zhong Y et al. Protective effects of apigenin against 3-MCPD-induced renal injury in rat. Chem Biol Interact 296:9-17 (2018). PubMed: 30107153
- Mercatelli E et al. Direct structural evidence of protein redox regulation obtained by in-cell NMR. Biochim Biophys Acta 1863:198-204 (2016). PubMed: 26589182