AB190251

Anti-COX16 抗体

Name: Anti-COX16 抗体 (ab190251) | Abcam
Brand: Abcam Limited
SKU: AB190251
Price: 89000 JPY
Availability: InStock

Anti-COX16 antibody

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(1 Publication)

Rabbit Polyclonal COX16 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus.

別名を表示する

C14orf112, HSPC203, PTD019, COX16, hCOX16

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue labeling COX16 in the cytoplasm and membrane of smooth muscle cells with ab190251 at a 1/200 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

Immunocytochemical analysis of MCF7 (Human breast adenocarcinoma cell line) whole cells labeling COX16 in the mitochondria with ab190251 at 2 μg/ml.

Supplier Data

Western blot - Anti-COX16 antibody (AB190251)

All lanes:

Western blot - Anti-COX16 antibody (ab190251) at 0.4 µg/mL

Lane 1:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 2:

U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate

Predicted band size: 12 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human COX16 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P0S2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

COX16 also known as cytochrome c oxidase assembly factor COX16 is a mitochondrial membrane protein with a molecular mass of approximately 16 kDa. This protein is expressed primarily in tissues with high energy demands such as skeletal muscles heart and brain. It resides in the inner mitochondrial membrane where it assists in the stabilization and assembly of cytochrome c oxidase an important component of the mitochondrial respiratory chain.

Biological function summary

COX16 plays a role in facilitating the assembly of cytochrome c oxidase which is a multi-subunit complex. This enzyme complex is responsible for the final step in the electron transport chain a process critical for ATP production in cells. COX16 contributes to the incorporation of subunit Cox1 into the growing enzyme complex ensuring proper enzyme functionality and cellular energy production.

Pathways

COX16 is integral to mitochondrial oxidative phosphorylation and respiratory chain complexes formation. It interacts closely with other proteins such as SURF1 and LRPPRC which are also involved in cytochrome c oxidase assembly. Dysfunction in these pathways can lead to impaired energy production and metabolic disturbances at the cellular level.

COX16 mutations have been linked to disorders like mitochondrial complex IV deficiency and Leigh syndrome. These diseases manifest with neurological and muscular symptoms due to impaired energy metabolism. COX16's dysfunction in these conditions often involves interactions with proteins like COX10 and COX2 further implicating the cytochrome oxidase complex's role in disease pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase (PubMed : 29355485, PubMed : 29381136, PubMed : 33169484). Promotes the insertion of copper into the active site of cytochrome c oxidase subunit II (MT-CO2/COX2) (PubMed : 29355485, PubMed : 29381136). Interacts specifically with newly synthesized MT-CO2/COX and its copper center-forming metallochaperones SCO1, SCO2 and COA6 (PubMed : 29381136). Probably facilitates MT-CO2/COX2 association with the MITRAC assembly intermediate containing MT-CO1/COX1, thereby participating in merging the MT-CO1/COX1 and MT-CO2/COX2 assembly lines (PubMed : 29381136).

See full target information COX16

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 161:27 PubMed39192374

2024

The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkui Jin,Wang Xu,Kunlin Yu,Cailu Luo,Xiaodan Luo,Tao Lian,Changshan Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COX16 抗体

Anti-COX16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX16 antibody (AB190251)

Immunocytochemistry/ Immunofluorescence - Anti-COX16 antibody (AB190251)

Western blot - Anti-COX16 antibody (AB190251)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

Abcam product promise