Anti-COX1 / Cyclooxygenase 1 抗体 [5F6/F4] (ab695)
![Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](https://www.abcam.co.jp/ps/products/0/ab695/Images/ab695-3642-anti-cox1-cyclooxygenase-1-antibody-5f6-f4-flow-cytometry.jpg "Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)")
Key features and details
- Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
製品の概要
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製品名
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
COX1 / Cyclooxygenase 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Human COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
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ポジティブ･コントロール
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
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特記事項
Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
FCS free -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
5F6/F4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab695の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
細胞内局在
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 19224 Mouse
- SwissProt: P22437 Mouse
- Unigene: 275434 Mouse
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別名
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
画像
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Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab695 は 22 報の論文で使用されています。
- Raihan MO et al. Characterization of prostanoids response to Bordetella pertussis antigen BscF and Tdap in LPS-challenged monocytes. Prostaglandins Leukot Essent Fatty Acids 182:102452 (2022). PubMed: 35690004
- Iwata Y et al. The inhibitory effects of Orengedokuto on inducible PGE2 production in BV-2 microglial cells. Heliyon 7:e07759 (2021). PubMed: 34458607
- Gui M et al. Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production. Exp Ther Med 22:1446 (2021). PubMed: 34721688
- Baranowska-Kuczko M et al. Vasoprotective Endothelial Effects of Chronic Cannabidiol Treatment and Its Influence on the Endocannabinoid System in Rats with Primary and Secondary Hypertension. Pharmaceuticals (Basel) 14:N/A (2021). PubMed: 34832902
- Korczyñska-Krawczyk P et al. The effect of levocetirizine and montelukast on clinical symptoms, serum level and skin expression of COX-1 and COX-2 enzymes in patients suffering from chronic autoimmune urticaria - a pilot study. Postepy Dermatol Alergol 37:73-80 (2020). PubMed: 32467688