Anti-CoREST2 / RCOR2 抗体 (ab37113)
Key features and details
- Rabbit polyclonal to CoREST2 / RCOR2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-CoREST2 / RCOR2 antibody -
製品の詳細
Rabbit polyclonal to CoREST2 / RCOR2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CoREST2/ RCOR2.
Immunogen の所有権に関して (Peptide available as ab37307.) -
ポジティブ･コントロール
- IHC-P: FFPE human testis tissue secions.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 58 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 58 kDa). |
|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May act as a component of a corepressor complex that represses transcription. -
配列類似性
Belongs to the CoREST family.
Contains 1 ELM2 domain.
Contains 2 SANT domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 283248 Human
- Entrez Gene: 104383 Mouse
- Entrez Gene: 305811 Rat
- Entrez Gene: 498485 Rat
- SwissProt: Q8IZ40 Human
- SwissProt: Q8C796 Mouse
- SwissProt: Q5FWT8 Rat
- Unigene: 98788 Human
see all -
別名
- 1A13 antibody
- co repressor of Rest antibody
- co-repressor of Rest antibody
see all
画像
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Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113)All lanes : Anti-CoREST2 / RCOR2 antibody (ab37113) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CoREST2 / RCOR2 antibody (ab37113)IHC image of CoREST/RCOR2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37113, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-CoREST2 / RCOR2 antibody (ab37113)This image is courtesy of an anonymous abreview.Anti-CoREST2 / RCOR2 antibody (ab37113) at 1/1000 dilution + MCF7 cells
Secondary
HRP conjugated donkey anti-rabbit at 1/2000 dilution.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab37113 は 5 報の論文で使用されています。
- Žemaitis K et al. The stem cell-supporting small molecule UM171 triggers Cul3-KBTBD4-mediated degradation of ELM2 domain-harboring proteins. J Biol Chem 299:104662 (2023). PubMed: 36997086
- Tsang SM et al. Sox11 regulates mammary tumour-initiating and metastatic capacity in Brca1-deficient mouse mammary tumour cells. Dis Model Mech 14:N/A (2021). PubMed: 33969421
- Oliemuller E et al. SOX11 promotes epithelial/mesenchymal hybrid state and alters tropism of invasive breast cancer cells. Elife 9:N/A (2020). PubMed: 32909943
- Xiong Y et al. Inhibiting the coregulator CoREST impairs Foxp3+ Treg function and promotes antitumor immunity. J Clin Invest 130:1830-1842 (2020). PubMed: 31917688
- Khan DH et al. RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing. Nucleic Acids Res N/A:N/A (2013). PubMed: 24234443