Anti-COPS3/CSN3 抗体 (ab12321)
Key features and details
- Rabbit polyclonal to COPS3/CSN3
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-COPS3/CSN3 antibody
COPS3/CSN3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to COPS3/CSN3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus
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免疫原
Synthetic peptide within Human COPS3/CSN3 aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9UNS2 -
ポジティブ･コントロール
- HEK 293T cells.
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特記事項
Centrifuge tube to remove product from lid.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab12321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/5000 - 1/25000. Predicted molecular weight: 51 kDa.
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| 特記事項 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/5000 - 1/25000. Predicted molecular weight: 51 kDa. |
ターゲット情報
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機能
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
組織特異性
Widely expressed. Expressed at high level in heart and skeletal muscle. -
配列類似性
Belongs to the CSN3 family.
Contains 1 PCI domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 416504 Chicken
- Entrez Gene: 507932 Cow
- Entrez Gene: 479528 Dog
- Entrez Gene: 8533 Human
- Entrez Gene: 26572 Mouse
- Entrez Gene: 100171418 Orangutan
- Entrez Gene: 287367 Rat
- Entrez Gene: 379813 Xenopus laevis
see all -
別名
- Constitutive photomorphogenic homolog subunit 3 antibody
- COP 9 (constitutive photomorphogenic) subunit 3 antibody
- COP 9 complex homolog subunit 3 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPS3/CSN3 antibody (ab12321)
Immunohisotchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling COPS3/CSN3 with ab12321 at 1/1000 (1µg/ml). Detection: DAB.
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Western blot - Anti-COPS3/CSN3 antibody (ab12321)All lanes : Anti-COPS3/CSN3 antibody (ab12321) at 0.04 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 51 kDa
Exposure time: 10 seconds -
Western blot - Anti-COPS3/CSN3 antibody (ab12321)Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Detection of Human CSN3 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
Detection: Chemiluminescence with a 10 second exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab12321 は 2 報の論文で使用されています。
- Olma MH et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 122:1035-44 (2009). WB ; Human . PubMed: 19295130
- Oh W et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). PubMed: 16624822