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Anti-COP1 抗体 [1E4] (ab56400)

Reviews (4)Q&A (1)References (15)
Western blot - Anti-COP1 antibody [1E4] (ab56400)
  • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)
  • Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)

Key features and details

  • Mouse monoclonal [1E4] to COP1
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-COP1 antibody [1E4]
    COP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1E4] to COP1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Recombinant fragment: CLRSFKGHIN EKNFVGLASN GDYIACGSEN NSLYLYYKGL SKTLLTFKFD TVKSVLDKDR KEDDTNEFVS AVCWRALPDG ESNVLIAANS QGTIKVLELV , corresponding to amino acids 632-732 of Human COP1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 30th April 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab56400の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
ICC/IF (1)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. 

ターゲット情報

  • 機能

    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.

  • 組織特異性

    Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 配列類似性

    Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.

  • ドメイン

    The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).

  • 細胞内局在

    Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Constitutive photomorphogenesis protein 1 homolog antibody
      • COP antibody
      • E3 ubiquitin-protein ligase RFWD2 antibody
      • hCOP1 antibody
      • Rfwd2 antibody
      • RFWD2_HUMAN antibody
      • ring finger and WD repeat domain 2 antibody
      • RING finger and WD repeat domain protein 2 antibody
      • RING finger protein 200 antibody
      see all

    画像

    • Western blot - Anti-COP1 antibody [1E4] (ab56400)
      Western blot - Anti-COP1 antibody [1E4] (ab56400)

      COP1 antibody (ab56400) at 1ug/lane + NIH/3T3 cell lysate at 25ug/lane.

      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)
      Immunocytochemistry/ Immunofluorescence - Anti-COP1 antibody [1E4] (ab56400)

      Immunofluorescence of ab56400 on NIH/3T3 cell [antibody concentration 10 ug/ml].

      This image was generated using the ascites version of the product.

    • Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)
      Flow Cytometry - Anti-COP1 antibody [1E4] (ab56400)

      Overlay histogram showing NIH3T3 cells stained with ab56400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56400, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COP1 antibody [1E4] (ab56400)

      IHC image of COP1 staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab56400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      This image was generated using the ascites version of the product.

    参考文献 (15)

    ab56400 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab56400 は 15 報の論文で使用されています。

    • Albino D  et al. Circulating extracellular vesicles release oncogenic miR-424 in experimental models and patients with aggressive prostate cancer. Commun Biol 4:119 (2021). PubMed: 33500545
    • Guo M  et al. Targeting RFWD2 as an Effective Strategy to Inhibit Cellular Proliferation and Overcome Drug Resistance to Proteasome Inhibitor in Multiple Myeloma. Front Cell Dev Biol 9:675939 (2021). PubMed: 33968945
    • Ding L  et al. Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis. Nat Metab 3:1648-1661 (2021). PubMed: 34903883
    • Yoshikawa R  et al. Development of synthetic microRNA-214 showing enhanced cytotoxicity and RNase resistance for treatment of canine hemangiosarcoma. Vet Comp Oncol 18:570-579 (2020). PubMed: 32072720
    • Xu Y  et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-COP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BEL-7402)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    40 µg
    Treatment
    80 µM Doxorubicin for 24hrs
    Specification
    BEL-7402
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. guoqing zhu

    Verified customer

    投稿 Jan 22 2016

    Western blot abreview for Anti-COP1 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (kidney)
    Loading amount
    20 µg
    Specification
    kidney
    Gel Running Conditions
    Reduced Denaturing (10-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 11 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-COP1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Specification
    HEK293T
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 1% triton in PBS (20 min)
    Blocking step
    BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 31 2012

    Western blot abreview for Anti-COP1 antibody

     Average
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cell)
    Loading amount
    50 µg
    Specification
    293T cell
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jun 08 2012

    Question

    we are considering purchasing your mAb against RFWD2 (catalog number ab56400).

    However, we would first like to inquire whether this antibody is indeed suitable for immunofluorescence microscopy of human cultured cells, like for example the HeLa cell line. We are asking because the IF micrograph presented in your datasheet shows murine 3T3 cells. Could you please let us know whether IFM images of human cells too are available that we may have a look at? Could you also let us know whether this antibody has already been mentioned in a publication so that we could look at those images too?
    Moreover, could you please tell us whether the immunoblot presented on you website shows the labelling of the endogenous RFWD2 from 3T3 cells or whether a lysate of cells transfected with an RFWD2 expression vector has been used for blotting.

    Finally, could you let us know whether this antibody has been quality-controlled by immunoblotting and IFM by your own company and whether or not the antibody and the corresponding images may possibly stem from another source.

    Thank you for your help.

    Read More
    Answer

    Vielen Dank für Ihre Anfrage.

    Bitte entschuldigen Sie die Verzögerung: ich hatte mich mit dem Labor, welches den Antikörper herstellt, in Verbindung gesetzt.

    Hier nun die Antworten bezüglich Ihrer Fragen:

    - der AK wurde am endogenen Mausprotein getestet
    - wir haben leider keine Abbildung in der ICC mit humanen Zellen
    - es wurde bisher nur das rekombinante humane Protein im WB getestet (das Datenblatt wird dem entsprechend aktualisiert)


    Falls Siees selbst testen möchten, ob der AK in humanen Zellen funktioniert, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von ab56400abdecken, und gegen eine erneute Bestellung eines weiteren primären Antikörpers von uns verrechnet werden.

    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab56400in humanen Zellentesten möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in humanen Zellen.

    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können einen anderen primären Antikörper zu dem gleichen Preis wie ab56400bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab56400bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

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