Anti-Contactin 1 抗体 (ab66265)
Key features and details
- Rabbit polyclonal to Contactin 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Contactin 1 antibody
Contactin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Contactin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Pig
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse Contactin 1.
Immunogen の所有権に関して (Peptide available as ab74597.) -
ポジティブ･コントロール
- WB: Human, Mouse and Rat Brain Tissue Lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab66265の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 113 kDa). |
追加情報
Is unsuitable for ICC/IF.
ターゲット情報
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機能
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. -
組織特異性
Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle. -
関連疾患
Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. -
配列類似性
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 281705 Cow
- Entrez Gene: 1272 Human
- Entrez Gene: 12805 Mouse
- Entrez Gene: 100521555 Pig
- Entrez Gene: 117258 Rat
- Omim: 600016 Human
- SwissProt: Q28106 Cow
- SwissProt: Q12860 Human
see all -
別名
- CNTN 1 antibody
- CNTN antibody
- Cntn1 antibody
see all
画像
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Western blot - Anti-Contactin 1 antibody (ab66265)All lanes : Anti-Contactin 1 antibody (ab66265) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 113 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Contactin 1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab66265 は 5 報の論文で使用されています。
- Gilhooley MJ et al. ON-bipolar cell gene expression during retinal degeneration: Implications for optogenetic visual restoration. Exp Eye Res 207:108553 (2021). PubMed: 33811915
- Wang B et al. Upregulation of contactin-1 expression promotes prostate cancer progression. Oncol Lett 19:1611-1618 (2020). PubMed: 32002038
- Smalley JL et al. Isolation and Characterization of Multi-Protein Complexes Enriched in the K-Cl Co-transporter 2 From Brain Plasma Membranes. Front Mol Neurosci 13:563091 (2020). PubMed: 33192291
- Chatterjee M et al. Contactin-1 Is Reduced in Cerebrospinal Fluid of Parkinson's Disease Patients and Is Present within Lewy Bodies. Biomolecules 10:N/A (2020). PubMed: 32806791
- Dembla M et al. Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis. EMBO Mol Med 10:N/A (2018). PubMed: 30266776