Anti-Connexin 45/GJA7/Cx45 抗体 [5B9.2] (ab78408)
Key features and details
- Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45
- Suitable for: Flow Cyt, ICC
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2]
Connexin 45/GJA7/Cx45 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICCmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Cow, Dog, Pig, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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エピトープ
Near C terminal, cytoplasmic. -
ポジティブ・コントロール
- ICC/IF: Connexin 45/GJA7/Cx45 transfected rat C6 cells. Flow Cyt: HeLa cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 17th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
5B9.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78408の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC |
Use at an assay dependent concentration.
|
特記事項 |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
配列類似性
Belongs to the connexin family. Gamma-type subfamily. -
細胞内局在
Cell membrane. Cell junction > gap junction. - Information by UniProt
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参照データベース
- Entrez Gene: 100689357 Chinese hamster
- Entrez Gene: 511119 Cow
- Entrez Gene: 490936 Dog
- Entrez Gene: 10052 Human
- Entrez Gene: 14615 Mouse
- Entrez Gene: 100048959 Pig
- Entrez Gene: 266706 Rat
- Omim: 608655 Human
see all -
別名
- Connexin 45 / GJA7 antibody
- Connexin 45 antibody
- Connexin-45 antibody
see all
画像
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ab78408 staining Connexin 45/GJA7/Cx45 transfected rat C6 cells by Immunocytochemistry.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab78408 は 9 報の論文で使用されています。
- Hatabi K et al. Inhibition of Gap Junctional Intercellular Communication Upregulates Pluripotency Gene Expression in Endogenous Pluripotent Muse Cells. Cells 11:N/A (2022). PubMed: 36078111
- Ye X et al. Study on Optimal Parameter and Target for Pulsed-Field Ablation of Atrial Fibrillation. Front Cardiovasc Med 8:690092 (2021). PubMed: 34621795
- Zhou E et al. Vascular smooth muscle cell phenotypic transition regulates gap junctions of cardiomyocyte. Heart Vessels 35:1025-1035 (2020). PubMed: 32270355
- Lee J et al. In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix. Nat Commun 11:4283 (2020). PubMed: 32883967
- Xiao H et al. Transcription factor Tbx18 induces the differentiation of c-kit+ canine mesenchymal stem cells (cMSCs) into SAN-like pacemaker cells in a co-culture model in vitro. Am J Transl Res 10:2511-2528 (2018). PubMed: 30210689
- Wan HJ et al. Propofol-induced vasodilation of mesenteric arterioles via BKCa channel and gap junction. Exp Ther Med 16:2960-2968 (2018). PubMed: 30233668
- Liu H et al. Role of gap junctions in the contractile response to agonists in the mesenteric resistance artery of rats with acute hypoxia. Mol Med Rep 15:1823-1831 (2017). PubMed: 28259990
- Dovmark TH et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells. Oncogene 36:4538-4550 (2017). WB . PubMed: 28368405
- Li D et al. Connexins and steroidogenesis in mouse Leydig cells. Can J Physiol Pharmacol 91:157-64 (2013). PubMed: 23458200