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AB135474

Anti-Connexin 45/GJA7/Cx45 抗体

Anti-Connexin 45/GJA7/Cx45 antibody

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(15 Publications)

Rabbit Polyclonal Connexin 45/GJA7/Cx45 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human GJC1.

別名を表示する

GJA7, GJC1, Gap junction gamma-1 protein, Connexin-45, Gap junction alpha-7 protein, Cx45

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 45/GJA7/Cx45 antibody (AB135474)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 45/GJA7/Cx45 antibody (AB135474)

Immunohistochemical analysis of paraffin embedded Human brain tissue labelling Connexin 45/GJA7/Cx45 with ab135474 at 1/50 dilution.

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody (AB135474)
  • WB

Unknown

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody (AB135474)

All lanes:

Western blot - Anti-Connexin 45/GJA7/Cx45 antibody (ab135474) at 1/500 dilution

All lanes:

Rat heart extracts at 30 µg

Predicted band size: 45 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Rat, Human

アプリケーション

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human GJC1. The exact immunogen used to generate this antibody is proprietary information.

P36383

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Connexin 45 also known as Cx45 or GJA7 is a gap junction protein that enables cellular communication by forming channels between adjacent cells. The channels regulate ion and small molecule passage which is critical for maintaining cellular homeostasis. Cx45 has a molecular weight of approximately 45 kDa. This protein expresses in various tissues including the heart brain and smooth muscle indicating its diverse physiological roles.
Biological function summary

Cx45 plays an integral role in intercellular communication by participating in the formation of gap junction channels. It functions as part of the connexin family forming homomeric and heteromeric hemichannels that dock with hemichannels on adjacent cells. This connection is essential for electronic coupling and synchronous activity especially in the cardiac and neural tissues where timely communication is necessary for function.

Pathways

Cx45 contributes significantly to the cardiac conduction system and neural transmission pathways. In cardiac tissues it coordinates with connexin 43 to control electrical signal propagation and regulate heartbeat. Cx45 participates in neural pathways by forming functional networks that support synaptic transmission and neuronal synchronization thereby interacting with proteins like connexin 36.

Mutations or dysregulation of Cx45 associate with cardiac arrhythmias and neurological disorders like epilepsy. In the heart Cx45 along with connexin 43 is essential for maintaining normal cardiac rhythm and disturbances can lead to arrhythmogenic conditions. In the nervous system Cx45 dysfunction can affect neural circuitry contributing to epileptic seizures. It is vital to investigate the role of Cx45 in these conditions for developing therapeutic strategies.

製品プロトコール

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ターゲットの情報

Structural component of the gap junction, a specialized intercellular structure consisting of a cluster of closely packed pairs of transmembrane channels, the connexons, that allow passage of small molecules and electrical signals between neighboring cells. Forms homotypic and heterotypic channels gated by transjunctional voltage (By similarity). Involved in intercellular innate immune signaling. Mediates translocation of 2',3'-cGAMP and 2',5'-oligoadenylates (2-5A) second messengers from virus-infected cells to macrophages and uninfected neighboring cells to propagate and amplify the antiviral immune response (PubMed : 24077100, PubMed : 31992625, PubMed : 40010341).
See full target information GJC1

文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39334940

2024

Connexin Expression Is Altered in the Eye Development of Mice: A Preliminary Study.

Applications

Unspecified application

Species

Unspecified reactive species

Ljubica Skelin,Anita Racetin,Nela Kelam,Marin Ogorevc,Ljubo Znaor,Mirna Saraga-Babić,Natalija Filipović,Yu Katsuyama,Zenon Pogorelić,Katarina Vukojević

iScience 27:109219 PubMed38469561

2024

Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mary E Mohr,Shuang Li,Allison M Trouten,Rebecca A Stairley,Patrick L Roddy,Chun Liu,Min Zhang,Henry M Sucov,Ge Tao

Frontiers in cellular neuroscience 17:1240916 PubMed37829672

2023

Orally administered boldine reduces muscle atrophy and promotes neuromuscular recovery in a rodent model of delayed nerve repair.

Applications

Unspecified application

Species

Unspecified reactive species

Justin C Burrell,Phuong T Vu,Owen J B Alcott,Carlos A Toro,Christopher Cardozo,D Kacy Cullen

Genes 14: PubMed36833374

2023

Expression of Connexins 37, 40 and 45, Pannexin 1 and Vimentin in Laryngeal Squamous Cell Carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Mizdrak,Maja Mizdrak,Anita Racetin,Braco Bošković,Benjamin Benzon,Merica Glavina Durdov,Katarina Vukojević,Natalija Filipović

Life (Basel, Switzerland) 12: PubMed36013400

2022

Effects of Obesity and Diabesity on Ventricular Muscle Structure and Function in the Zucker Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Sultan,Ernest Adeghate,Bright Starling Emerald,Muhammad A Qureshi,Saeed Tariq Minhas,Frank Christopher Howarth

International journal of molecular sciences 23: PubMed35682927

2022

A Lack of GD3 Synthase Leads to Impaired Renal Expression of Connexins and Pannexin1 in Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Meter,Anita Racetin,Katarina Vukojević,Marta Balog,Vedrana Ivić,Milorad Zjalić,Marija Heffer,Natalija Filipović

International journal of molecular sciences 23: PubMed35682601

2022

The Expression of Connexin 37, 40, 43, 45 and Pannexin 1 in the Early Human Retina and Choroid Development and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Matea Žužul,Mirela Lozić,Natalija Filipović,Samir Čanović,Ana Didović Pavičić,Joško Petričević,Nenad Kunac,Violeta Šoljić,Mirna Saraga-Babić,Suzana Konjevoda,Katarina Vukojevic

Biomedicines 10: PubMed35327391

2022

The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1-/-) Mice and Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Josip Lesko,Pejana Rastović,Josip Mišković,Violeta Šoljić,Vlatka Paštar,Zdenka Zovko,Natalija Filipović,Yu Katsuyama,Mirna Saraga-Babić,Katarina Vukojević

International journal of molecular sciences 22: PubMed34639052

2021

Connexin Expression Is Altered in Liver Development of () Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vlatka Paštar,Mirela Lozić,Nela Kelam,Natalija Filipović,Branka Bernard,Yu Katsuyama,Katarina Vukojević

International journal of molecular sciences 22: PubMed33525532

2021

Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- () Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mirela Lozić,Natalija Filipović,Marija Jurić,Ivona Kosović,Benjamin Benzon,Ivana Šolić,Nela Kelam,Anita Racetin,Koichiro Watanabe,Yu Katsuyama,Masaki Ogata,Mirna Saraga-Babić,Katarina Vukojević
View all publications

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