Anti-COMP/Cartilage oligomeric matrix protein 抗体 [EPR25364-6] (ab300555)

製品名

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6]
COMP/Cartilage oligomeric matrix protein 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR25364-6] to COMP/Cartilage oligomeric matrix protein
由来種

Rabbit
アプリケーション

適用あり: IHC-Fr, WB, IHC-P, IPmore details
適用なし: Flow Cyt (Intra) or ICC/IF
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- IHCFr: Mouse and Rat P0 articular cartilage fresh frozen tissues. WB: Mouse articular cartilage, Rat articular cartilage, Human articular cartilage tissue lysates. IP: Human skeletal muscle tissue lysate. IHC-P: Mouse cartilage, Mouse skeletal muscle, Human skeletal muscle FFPE tissue sections.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR25364-6
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (BSA and Azide free) (ab300556)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab300555の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-Fr		1/500. Not Applicable.
WB		1/1000.
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.

特記事項
IHC-Fr 1/500. Not Applicable.
WB 1/1000.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.

追加情報

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

機能

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7.
組織特異性

Abundantly expressed in the chondrocyte extracellular matrix, and is also found in bone, tendon, ligament and synovium and blood vessels. Increased amounts are produced during late stages of osteoarthritis in the area adjacent to the main defect.
関連疾患

Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1) [MIM:132400]. EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types.
Defects in COMP are the cause of pseudoachondroplasia (PSACH) [MIM:177170]. PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood.
配列類似性

Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 8 TSP type-3 repeats.
発生段階

Present during the earliest stages of limb maturation and is later found in regions where the joints develop.
ドメイン

The cell attachment motif mediates the attachment to chondrocytes. It mediates the induction of both the IAP family of survival proteins and the antiapoptotic response.
The TSP C-terminal domain mediates interaction with FN1 and ACAN.
細胞内局在

Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accession P49747 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 1311 Human
- Entrez Gene: 12845 Mouse
- Entrez Gene: 25304 Rat
- Omim: 600310 Human
- SwissProt: P49747 Human
- SwissProt: Q9R0G6 Mouse
- SwissProt: P35444 Rat
- Unigene: 1584 Human
- Unigene: 45071 Mouse
- Unigene: 10343 Rat
see all
別名
- cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) antibody
- Cartilage oligomeric matrix protein antibody
- Cartilage oligomeric matrix protein precursor antibody
- COMP antibody
- COMP_HUMAN antibody
- EDM 1 antibody
- EDM1 antibody
- EPD 1 antibody
- EPD1 antibody
- Epiphyseal dysplasia 1 antibody
- Epiphyseal dysplasia 1 multiple antibody
- Epiphyseal dysplasia multiple 1 antibody
- MED antibody
- MGC13181 antibody
- MGC149768 antibody
- PSACH antibody
- pseudoachondroplasia (epiphyseal dysplasia 1, multiple) antibody
- Pseudoachondroplasia antibody
- THBS 5 antibody
- THBS5 antibody
- Thrombospondin 5 antibody
- Thrombospondin-5 antibody
- Thrombospondin5 antibody
- TSP5 antibody
see all

Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

All lanes : Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/1000 dilution

Lane 1 : Mouse articular cartilage tissue lysate at 20 µg
Lane 2 : Mouse liver tissue lysate at 40 µg
Lane 3 : Rat articar cartilage tissue lysate at 20 µg
Lane 4 : Rat liver tissue lysate at 40 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Observed band size: 100,82 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 24917676).

The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID: 9143347).

Negative control: liver (PMID: 14748877)

Exposure time: 15 seconds
Western blot - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (ab300555) at 1/5000 dilution + Human articular cartilage tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/10000 dilution

Observed band size: 100,82 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 24917676).

The band at 82 KDa likely represents the unglycosylated form of the COMP monomer (PMID: 9143347).

Exposure time: 15 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 dilution (0.246 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse cartilage (PMID: 33668140, PMID:16542502 ). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 dilution (0.246 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on mouse skeletal muscle (PMID: 19808781). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 dilution (0.246 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse liver (PMID: 14748877). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 dilution (0.246 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on human skeletal muscle (PMID: 19808781). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/2000 dilution (0.246 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on human liver (PMID: 14748877). The section was incubated with ab300555 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.98µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on mouse P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.98µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on mouse liver (PMID: 14748877). is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 articular cartilage (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.98 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on rat P0 articular cartilage is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling COMP/Cartilage oligomeric matrix protein with ab300555 at 1/500 (0.98 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on rat liver (PMID: 14748877) is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Human skeletal muscle tissue lysate 10 µg with ab300555 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human skeletal muscle tissue lysate 10 µg

Lane 2: ab300555 IP in Human skeletal muscle tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300555 in human skeletal muscle tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 76 seconds
Immunoprecipitation - Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

COMP/Cartilage oligomeric matrix protein was immunoprecipitated from 0.35 mg Mouse articular cartilage tissue lysate 10 µg with ab300555 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300555 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Mouse articular cartilage tissue lysate 10 µg

Lane 2: ab300555 IP in Mouse articular cartilage tissue lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300555 in mouse articular cartilage tissue lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST

Exposure time: 6 seconds
Anti-COMP/Cartilage oligomeric matrix protein antibody [EPR25364-6] (AB300555)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

発生段階

ドメイン

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

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